Frateuria soli sp. nov. and Frateuria edaphi sp. nov., isolated from greenhouse soil

Abstract

Two bacterial strains, designated 5GH9-11^T and 5GH9-34^T, were isolated from greenhouse soil sampled in Wanju-gun, Jeollabuk-do, Republic of Korea. Both strains formed yellow colonies and were aerobic, rod-shaped and flagellated. The 16S rRNA gene sequence similarity between 5GH9-11^T and 5GH9-34^T was 98.6 %. Strain 5GH9-11^T showed the highest sequence similarities to Dyella thiooxydans ATSB10^T (98.1 %) and Frateuria aurantia DSM 6220^T (97.7 %) while strain 5GH9-34^T revealed the highest sequence similarity to F. aurantia DSM 6220^T (98.3 %) and D. thiooxydans ATSB10^T (98.3 %). Phylogenetic analysis on the basis of the 16S rRNA gene sequence showed that strains 5GH9-11^T and 5GH9-34^T formed a robust cluster with Frateuria flava MAH-13^T and Frateuria terrea NBRC 104236^T. The phylogenomic tree also showed that strains 5GH9-11^T and 5GH9-34^T formed a robust cluster with F. terrea DSM 26515^T and F. flava MAH-13^T. Strain 5GH9-11^T showed the highest orthologous average nucleotide identity (OrthoANI; 88.5 %) and digital DNA-DNA hybridization (dDDH) values (35.5 %) with F. flava MAH-13^T, and strain 5GH9-34^T revealed highest OrthoANI (88.1 %) and dDDH (34.2 %) values with F. flava MAH-13^T. The orthoANI and dDDH values between strain 5GH9-11^T and 5GH9-34^T were 87.7 and 33.9 %, respectively. Their major respiratory quinone was ubiquinone 8, and the major cellular fatty acids were iso-C_{16 : 0}, summed feature 9 (iso-C_{17 : 1} ω9c and/or C_{16 : 0} 10-methyl) and iso-C_{15 : 0}. The major polar lipids of both strains were composed of large or moderate amounts of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, an unidentified aminolipid and an unidentified aminophospholipid. Based on these data, strains 5GH9-11^T and 5GH9-34^T should represent two independent novel species of Frateuria, for which the names Frateuria soli sp. nov. (type strain 5GH9-11^T=KACC 16943^T=JCM 35197^T) and Frateuria edaphi sp. nov. (type strain 5GH9-34^T=KACC 16945^T=JCM 35198^T) are proposed.

Keywords: Frateuria edaphi sp. nov.; Frateuria soli sp. nov.; phylogenetic tree; soil.