Protocol to assemble metal-phenolic framework nanoparticles based on polyphenol-mediated biomimetic mineralization for wound healing in rats

Xiaolong Zhan; Zhengbo Wen; Yuxin Chen; Jiaxin Feng; Ke Liang; Liecong Xue; Peiying He; Honglin Chen; Wei Zhu

doi:10.1016/j.xpro.2023.102131

Protocol to assemble metal-phenolic framework nanoparticles based on polyphenol-mediated biomimetic mineralization for wound healing in rats

STAR Protoc. 2023 Mar 17;4(1):102131. doi: 10.1016/j.xpro.2023.102131. Epub 2023 Feb 25.

Authors

Xiaolong Zhan¹, Zhengbo Wen², Yuxin Chen², Jiaxin Feng³, Ke Liang⁴, Liecong Xue⁴, Peiying He⁴, Honglin Chen⁵, Wei Zhu⁶

Affiliations

¹ MOE International Joint Research Laboratory on Synthetic Biology and Medicines, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, Guangdong 510006, China. Electronic address: bizxl@mail.scut.edu.cn.
² Laboratory of Stem Cells and Translational Medicine, Institutes for Life Sciences and School of Medicine, South China University of Technology, Guangzhou, Guangdong 510006, China.
³ Guangdong Provincial Key Laboratory of Clinical Pharmacology, Guangdong Provincial People's Hospital, Guangzhou, Guangdong 510080, China.
⁴ MOE International Joint Research Laboratory on Synthetic Biology and Medicines, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, Guangdong 510006, China.
⁵ Medical Research Institute, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou 510080, China. Electronic address: chenhl@scut.edu.cn.
⁶ MOE International Joint Research Laboratory on Synthetic Biology and Medicines, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, Guangdong 510006, China. Electronic address: zhuwei86@scut.edu.cn.

Abstract

Here, we present a protocol for controllable biomimetic mineralization at the nano-scale, simulating natural ion-enriched sedimentary mineralization. We describe steps for treatment of metal-organic frameworks with polyphenol-mediated stabilized mineralized precursor solution. We then detail their use as templates to assemble metal-phenolic frameworks (MPFs) with mineralized layers. Furthermore, we demonstrate the therapeutic benefits of MPF delivery by hydrogel to the full-thickness skin defect model in rats. For complete details on the use and execution of this protocol, please refer to Zhan et al. (2022).¹.

Keywords: Biotechnology and Bioengineering; Chemistry; Health Sciences; Material Sciences; Microscopy; Model Organisms; Tissue Engineering.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Biomimetics
Nanoparticles*
Phenols
Polyphenols* / pharmacology
Rats
Wound Healing

Substances

Polyphenols
Phenols