Quantify permeability using on-a-chip models in high-throughput applications

Camilla Soragni; Tessa Vergroesen; Nynke Hettema; Gwenaëlle Rabussier; Henriëtte L Lanz; Sebastian J Trietsch; Leon J de Windt; Chee P Ng

doi:10.1016/j.xpro.2023.102051

Quantify permeability using on-a-chip models in high-throughput applications

STAR Protoc. 2023 Mar 17;4(1):102051. doi: 10.1016/j.xpro.2023.102051. Epub 2023 Jan 20.

Authors

Camilla Soragni¹, Tessa Vergroesen², Nynke Hettema², Gwenaëlle Rabussier³, Henriëtte L Lanz², Sebastian J Trietsch², Leon J de Windt⁴, Chee P Ng⁵

Affiliations

¹ MIMETAS BV, De Limes 7, 2342 DH Oegstgeest, the Netherlands; Department of Cardiology, Maastricht University, P. Debyelaan 25, 6229 HX Maastricht, the Netherlands. Electronic address: c.soragni@maastrichtuniversity.nl.
² MIMETAS BV, De Limes 7, 2342 DH Oegstgeest, the Netherlands.
³ MIMETAS BV, De Limes 7, 2342 DH Oegstgeest, the Netherlands; Department of Cardiology, Maastricht University, P. Debyelaan 25, 6229 HX Maastricht, the Netherlands.
⁴ Department of Cardiology, Maastricht University, P. Debyelaan 25, 6229 HX Maastricht, the Netherlands.
⁵ MIMETAS BV, De Limes 7, 2342 DH Oegstgeest, the Netherlands. Electronic address: c.ng@mimetas.com.

Abstract

Traditionally, to quantify permeability of a biological barrier, the initial slope is used, based on the assumption of sink condition (concentration of the donor is constant, and the receiver increases less than 10%). With on-a-chip barrier models, this assumption fails in cell-free or leaky conditions, which requires the use of the exact solution. To encounter a time delay from performing the assay and acquiring the data, we present a protocol with the exact equation modified to incorporate a time offset.

Keywords: Biotechnology and bioengineering; Cell-based Assays; High-throughput Screening; Microscopy.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biological Assay*
Lab-On-A-Chip Devices*
Permeability