Protocol for modulation of the serotonergic DR-PBC neural circuit to prevent SUDEP in the acoustic and PTZ-induced DBA/1 mouse models of SUDEP

YuLing Wang; Qing Xu; Qian Yu; LeYuan Gu; HaiXiang Ma; Yue Shen; XiTing Lian; WeiHui Shao; JiaXuan Gu; Lu Liu; HongHai Zhang

doi:10.1016/j.xpro.2023.102129

Protocol for modulation of the serotonergic DR-PBC neural circuit to prevent SUDEP in the acoustic and PTZ-induced DBA/1 mouse models of SUDEP

STAR Protoc. 2023 Mar 17;4(1):102129. doi: 10.1016/j.xpro.2023.102129. Epub 2023 Feb 20.

Authors

YuLing Wang¹, Qing Xu², Qian Yu¹, LeYuan Gu¹, HaiXiang Ma¹, Yue Shen¹, XiTing Lian², WeiHui Shao¹, JiaXuan Gu¹, Lu Liu², HongHai Zhang³

Affiliations

¹ Department of Anesthesiology, the Fourth Clinical School of Medicine, Zhejiang Chinese Medical University, Hangzhou 310006, China.
² Department of Anesthesiology, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine, Hangzhou 310006, China.
³ Department of Anesthesiology, the Fourth Clinical School of Medicine, Zhejiang Chinese Medical University, Hangzhou 310006, China; Department of Anesthesiology, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine, Hangzhou 310006, China; Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou 310006, China. Electronic address: zhanghonghai_0902@163.com.

Abstract

The dorsal raphe nucleus (DR) and the pre-Bötzinger complex (PBC) may play an important role in regulating seizure-induced respiratory arrest (S-IRA), the main contributor to sudden unexpected death in epilepsy. Here, we describe pharmacological, optogenetic, and retrograde labeling approaches to specifically modulate the DR to PBC serotonergic pathway. We detail steps for implanting optical fibers and viral infusion into DR and PBC regions and optogenetic techniques for exploring the role of 5-hydroxytryptophan (5-HT) neural circuit of DR-PBC in S-IRA. For complete details on the use and execution of this protocol, please refer to Ma et al. (2022).¹.

Keywords: Health Sciences; Neuroscience.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acoustics
Animals
Death, Sudden / prevention & control
Mice
Mice, Inbred DBA
Seizures / chemically induced
Seizures / metabolism
Sudden Unexpected Death in Epilepsy* / prevention & control