LncRNA PANTR1 is Associated with Poor Prognostic and Suppresses Apoptosis in Glioma

Fei Shi; Jie Hu; Ping Zheng; Yisong Lv; Hongyu Liu; Guiyun Zhang; Hongyu Jiang

doi:10.1155/2023/8537036

LncRNA PANTR1 is Associated with Poor Prognostic and Suppresses Apoptosis in Glioma

J Oncol. 2023 Feb 20:2023:8537036. doi: 10.1155/2023/8537036. eCollection 2023.

Authors

Fei Shi¹, Jie Hu², Ping Zheng³, Yisong Lv⁴, Hongyu Liu^{5

6

7}, Guiyun Zhang¹, Hongyu Jiang⁸

Affiliations

¹ Department of Neurovascular Intervention and Neurosurgery, Clinical Center of Neuroscience, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
² Department of Neurosurgery, Jiading Branch of Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
³ Department of Neurosurgery, Shanghai Pudong New Area People's Hospital, Shanghai, China.
⁴ School of Continuing Education, Shanghai Jiao Tong University, Shanghai, China.
⁵ Department of Neurosurgery, First Medical Center of Chinese PLA General Hospital, Beijing, China.
⁶ Department of Neurosurgery, Hainan Hospital of Chinese PLA General Hospital, Beijing, China.
⁷ Medical School of Chinese PLA, Beijing, China.
⁸ Department of Anesthesiology, Wuxi Second People's Hospital, Wuxi, Jiangsu Province, China.

Abstract

Glioma is the most common tumor in the central nervous system. High-grade gliomas confer a poor prognosis, being a serious health and economic burden. Current literature suggests the important role of long noncoding RNA (lncRNA) in mammals, especially in tumorigenesis of various tumors. The functions of lncRNA POU3F3 adjacent noncoding transcript 1 (PANTR1) have been investigated in hepatocellular carcinoma but remain yet unclear in gliomas. We evaluated the role of PANTR1 in glioma cells using published data from The Cancer Genome Atlas (TCGA), then validated it by ex vivo experiments. To investigate the potential cellular mechanism of different levels of PANTR1 expression in glioma cells, we used siRNA-mediated knockdown in low-grade (grade II) cell lines and GBM (grade IV) cell lines (SW1088 and SHG44, respectively). On the molecular level, low expression of PANTR1 caused significantly reduced glioma cell viability and enhanced cell death. Moreover, we identified the importance of PANTR1 expression for cell migration in both cell lines, a critical foundation for invasiveness in recurrent gliomas. In conclusion, this study provides the first evidence that PANTR1 has a relevant role in human glioma by influencing cell viability and cell death.