Structural characteristics and antioxidant and hypoglycemic activities of a heteropolysaccharide from Anemarrhena asphodeloides Bunge

Juncheng Chen; Lin Li; Xia Zhang; Yuan Zhang; Qingsong Zheng; Meijuan Lan; Bing Li

doi:10.1016/j.ijbiomac.2023.123843

Structural characteristics and antioxidant and hypoglycemic activities of a heteropolysaccharide from Anemarrhena asphodeloides Bunge

Int J Biol Macromol. 2023 May 1:236:123843. doi: 10.1016/j.ijbiomac.2023.123843. Epub 2023 Feb 27.

Authors

Juncheng Chen¹, Lin Li², Xia Zhang³, Yuan Zhang⁴, Qingsong Zheng³, Meijuan Lan³, Bing Li⁵

Affiliations

¹ School of Food Science and Engineering, Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Engineering Research Center of Starch and Plant Protein Deep Processing, Ministry of Education, South China University of Technology, Guangzhou 510640, China; International School of Public Health and One Health, Hainan Medical University, Haikou, Hainan 571199, China.
² School of Food Science and Engineering, Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Engineering Research Center of Starch and Plant Protein Deep Processing, Ministry of Education, South China University of Technology, Guangzhou 510640, China; School of Chemical Engineering and Energy Technology, Dongguan University of Technology, Dongguan 523808, China.
³ School of Food Science and Engineering, Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Engineering Research Center of Starch and Plant Protein Deep Processing, Ministry of Education, South China University of Technology, Guangzhou 510640, China.
⁴ Guangdong Provincial Institute of Sports Science, Guangzhou 510640, China.
⁵ School of Food Science and Engineering, Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Engineering Research Center of Starch and Plant Protein Deep Processing, Ministry of Education, South China University of Technology, Guangzhou 510640, China. Electronic address: bli@scut.edu.cn.

PMID: 36858093
DOI: 10.1016/j.ijbiomac.2023.123843

Abstract

In this study, an acid polysaccharide (AABP-1B) was extracted from the rhizome of Anemarrhena asphodeloides Bunge and purified using 60 % alcohol precipitation and DEAE-52 cellulose. The molecular weight of AABP-1B was 105 kDa, and it consisted of mannose (Man), rhamnose (Rha), galacturonic acid (GalA), glucose (Glc), galactose (Gal), and arabinose (Ara) in a ratio of 6.3:1.3:1.1:0.2:0.4:0.7. Methylation and NMR analyses revealed that the backbone of AABP-1 consists of 4)-β-D-Manp-(1 and 4)-2-O-acetyl-β-D-Manp-(1. In addition, the biological activity assays showed that AABP-1B not only displays potential antioxidant activity but also exhibits the α-glucosidase and α-amylase inhibitory effect. Moreover, AABP-1B enhanced glucose consumption and glycogen synthesis in insulin-resistant (IR) HepG2 cells. These results suggest that AABP-1B has potential hypoglycemic activity.

Keywords: Anemarrhena asphodeloides Bunge; Hypoglycemic effect; Polysaccharide.

MeSH terms

Anemarrhena* / chemistry
Antioxidants / pharmacology
Glucose
Humans
Hypoglycemic Agents* / chemistry
Hypoglycemic Agents* / pharmacology
Polysaccharides / chemistry
Polysaccharides / pharmacology

Substances

Hypoglycemic Agents
Antioxidants
Polysaccharides
Glucose