Protocol to engineer nanofilms embedded lipid nanoparticles for controlled and targeted drug delivery (NECTAR)

STAR Protoc. 2023 Mar 17;4(1):101685. doi: 10.1016/j.xpro.2022.101685. Epub 2023 Feb 28.

Abstract

We present a protocol to engineer a substrate-mediated delivery platform comprising hyaluronic acid-coated lipid nanoparticles (HALNPs) embedded into polyelectrolyte multilayer (PEM) films. This platform allows controlled spatiotemporal release of lipid nanoparticles (LNP) by embedding them within the polyelectrolyte multilayer films matrix. HALNP conjugate with antibodies also adds the ability for targeted delivery. The use of LNP enables this platform to encapsulate both hydrophobic and hydrophilic drugs. This platform can easily be reproduced and utilized for various biomedical drug delivery applications. For complete details on the use and execution of this protocol, please refer to Hayward et al. (2015, 2016a, 2016b), Hayward and Kidambi (2018), and Kidambi and Hayward (2022).

Keywords: Biotechnology and bioengineering; Material sciences.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Drug Delivery Systems* / methods
  • Liposomes
  • Plant Nectar*
  • Polyelectrolytes / chemistry

Substances

  • Lipid Nanoparticles
  • Plant Nectar
  • Polyelectrolytes
  • Liposomes