This paper reviews the research progress on live-cell super-resolution fluorescence microscopy, discusses the current research status and hotspots in this field, and summarizes the technological application of super-resolution fluorescence microscopy for live-cell imaging. To date, this field has gained progress in numerous aspects. Specifically, the structured illumination microscopy, stimulated emission depletion microscopy, and the recently introduced minimal photon fluxes microscopy are the current research hotspots. According to the current progress in this field, future development trend is likely to be largely driven by artificial intelligence as well as advances in fluorescent probes and relevant labelling methods.
本文系统综述了活细胞超分辨荧光显微成像技术相关研究进展，探讨了领域内研究现状及热点问题，并归纳总结了超分辨技术在活细胞成像中的应用。本文具体概括了该技术在相关领域取得的多方面研究进展，其中结构光照明显微术、受激发射损耗显微术以及最低光子通量显微术是当前研究的热点和前沿。根据当前领域内的发展态势，本文预计未来基于人工智能和荧光探针及其标记方法的创新将是该领域未来一段时间的重点发展方向。.
Keywords: Live-cell; Minimal photon fluxes; Stimulated emission depletion microscopy; Structured illumination microscopy; Super-resolution fluorescence microscopy.