In vitro bioaccessibility evaluation of chlorophyll pigments in single and binary carriers

Israel Emiezi Agarry; Desheng Ding; Yunchang Li; Zihan Jin; Huiling Deng; Jiang Hu; Tian Cai; Jianquan Kan; Kewei Chen

doi:10.1016/j.foodchem.2023.135757

In vitro bioaccessibility evaluation of chlorophyll pigments in single and binary carriers

Food Chem. 2023 Jul 30:415:135757. doi: 10.1016/j.foodchem.2023.135757. Epub 2023 Feb 24.

Authors

Israel Emiezi Agarry¹, Desheng Ding², Yunchang Li², Zihan Jin², Huiling Deng³, Jiang Hu³, Tian Cai⁴, Jianquan Kan⁵, Kewei Chen⁶

Affiliations

¹ College of Food Science, Southwest University, 2. Tiansheng Road, Beibei, Chongqing 400715, PR China; Laboratory of Quality & Safety Risk Assessment for Agro-products on Storage and Preservation (Chongqing), Ministry of Agriculture and Rural Affairs of the People's Republic of China, Chongqing 400715, PR China; China-Hungary Cooperative Centre for Food Science, Chongqing 400715, PR China.
² College of Food Science, Southwest University, 2. Tiansheng Road, Beibei, Chongqing 400715, PR China; Chongqing Key Laboratory of Specialty Food Co-built by Sichuan and Chongqing, Chongqing 400715, PR China.
³ Chongqing Chongke Inspection & Testing Co., Ltd, Building B, No. 2, Yangliu Road, 14 North New Area, Chongqing 401121, PR China.
⁴ School of Chemistry and Chemical Engineering, Southwest University, 2. Tiansheng Road, Beibei, Chongqing 400715, PR China.
⁵ College of Food Science, Southwest University, 2. Tiansheng Road, Beibei, Chongqing 400715, PR China; Laboratory of Quality & Safety Risk Assessment for Agro-products on Storage and Preservation (Chongqing), Ministry of Agriculture and Rural Affairs of the People's Republic of China, Chongqing 400715, PR China; China-Hungary Cooperative Centre for Food Science, Chongqing 400715, PR China; Chongqing Key Laboratory of Specialty Food Co-built by Sichuan and Chongqing, Chongqing 400715, PR China. Electronic address: kanjianquan@163.com.
⁶ College of Food Science, Southwest University, 2. Tiansheng Road, Beibei, Chongqing 400715, PR China; Laboratory of Quality & Safety Risk Assessment for Agro-products on Storage and Preservation (Chongqing), Ministry of Agriculture and Rural Affairs of the People's Republic of China, Chongqing 400715, PR China; China-Hungary Cooperative Centre for Food Science, Chongqing 400715, PR China; Chongqing Key Laboratory of Specialty Food Co-built by Sichuan and Chongqing, Chongqing 400715, PR China. Electronic address: chenkewei@swu.edu.cn.

PMID: 36854242
DOI: 10.1016/j.foodchem.2023.135757

Abstract

Chlorophyll was extracted and microencapsulated using different carrier agents. Subsequently, in vitro digestion was performed, and the bioaccessibility of chlorophyll in the different encapsulation systems was carried out. The zeta potential, particle size, and PDI were significantly modified after the micellarization of digested microcapsules. I-W-Chl presented with the highest total chlorophyll recovery and micellarization rate of 54% and 43%, respectively. In the aqueous micellar fraction, the different encapsulation systems had total chlorophylls, pheophytins, and pheophorbides ranging from 13 to 49%, 42 - 77%, and 3 - 22% respectively. The bioaccessibility of total chlorophyll pigment ranging from 7% to 20% is given in the following order: I-W-Chl > WPI-Chl > Z-Chl > Ca-Chl > SCChl^V > SCChl^C. The result established in this study shows that the carrier agent type could inhibit or mediate the bioaccessibility of chlorophyll with the potential to be an efficient delivery system for health promoting compounds.

Keywords: Bioaccessibility; Chlorophyll; Encapsulation; In vitro digestion; Micellarization; Microcapsules.

MeSH terms

Chlorophyll*
Pheophytins*

Substances

Chlorophyll
Pheophytins