Synthesis of Erythrocyte Nanodiscs for Bacterial Toxin Neutralization

Lei Sun; Dan Wang; Ilkoo Noh; Ronnie H Fang; Weiwei Gao; Liangfang Zhang

doi:10.1002/anie.202301566

Synthesis of Erythrocyte Nanodiscs for Bacterial Toxin Neutralization

Angew Chem Int Ed Engl. 2023 May 15;62(21):e202301566. doi: 10.1002/anie.202301566. Epub 2023 Mar 20.

Authors

Lei Sun¹, Dan Wang¹, Ilkoo Noh¹, Ronnie H Fang¹, Weiwei Gao¹, Liangfang Zhang¹

Affiliation

¹ Department of NanoEngineering, Chemical Engineering Program, and Moores Cancer Center, University of California San Diego, La Jolla, CA-92093, USA.

PMID: 36853913
DOI: 10.1002/anie.202301566

Abstract

Nanodiscs are a compelling nanomedicine platform due to their ultrasmall size and distinct disc shape. Current nanodisc formulations are made primarily with synthetic lipid bilayers and proteins. Here, we report a cellular nanodisc made with human red blood cell (RBC) membrane (denoted "RBC-ND") and show its effective neutralization against bacterial toxins. In vitro, RBC-ND neutralizes the hemolytic activity and cytotoxicity caused by purified α-toxin or complex whole secreted proteins (wSP) from methicillin-resistant Staphylococcus aureus bacteria. In vivo, RBC-ND confers significant survival benefits for mice intoxicated with α-toxin or wSP in both therapeutic and prevention regimens. Moreover, RBC-ND shows good biocompatibility and biosafety in vivo. Overall, RBC-ND distinguishes itself by inheriting the biological functions of the source cell membrane for bioactivity. The design strategy of RBC-ND can be generalized to other types of cell membranes for broad applications.

Keywords: Bacterial Toxin; Cell Membrane; Cellular Nanodisc; Detoxification; Nanomedicine.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Animals
Bacterial Toxins*
Erythrocyte Membrane
Erythrocytes
Humans
Lipid Bilayers
Methicillin-Resistant Staphylococcus aureus*
Mice

Substances

Bacterial Toxins
Lipid Bilayers