Characterization of a Recombinant Thermostable Newcastle Disease Virus (NDV) Expressing Glycoprotein gB of Infectious Laryngotracheitis Virus (ILTV) Protects Chickens against ILTV Challenge

Zhe Zeng; Yan He; Zichen Wang; Lun Yao; Li Li; Yu Shang; Hongcai Wang; Rongrong Zhang; Huabin Shao; Qingping Luo; Guoyuan Wen

doi:10.3390/v15020500

Characterization of a Recombinant Thermostable Newcastle Disease Virus (NDV) Expressing Glycoprotein gB of Infectious Laryngotracheitis Virus (ILTV) Protects Chickens against ILTV Challenge

Viruses. 2023 Feb 11;15(2):500. doi: 10.3390/v15020500.

Authors

Zhe Zeng^{1

2}, Yan He^{1

2}, Zichen Wang^{1

2}, Lun Yao^{1

2}, Li Li^{1

2}, Yu Shang^{1

2}, Hongcai Wang^{1

2}, Rongrong Zhang^{1

2}, Huabin Shao^{1

2}, Qingping Luo^{1

2

3}, Guoyuan Wen^{1

2}

Affiliations

¹ Key Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture and Rural Affairs), Institute of Animal Husbandry and Veterinary, Hubei Academy of Agricultural Sciences, Wuhan 430064, China.
² Hubei Provincial Key Laboratory of Animal Pathogenic Microbiology, Institute of Animal Husbandry and Veterinary, Hubei Academy of Agricultural Sciences, Wuhan 430064, China.
³ Hubei Hongshan Laboratory, Wuhan 430064, China.

Abstract

Infectious laryngotracheitis (ILT) and Newcastle disease (ND) are two important avian diseases that have caused huge economic losses to the poultry industry worldwide. Newcastle disease virus (NDV) has been used as a vector in the development of vaccines and gene delivery. In the present study, we generated a thermostable recombinant NDV (rNDV) expressing the glycoprotein gB (gB) of infectious laryngotracheitis virus (ITLV) based on the full-length cDNA clone of the thermostable TS09-C strain. This thermostable rNDV, named rTS-gB, displayed similar thermostability, growth kinetics, and pathogenicity compared with the parental TS09-C virus. The immunization data showed that rTS-gB induced effective ILTV- and NDV-specific antibody responses and conferred immunization protection against ILTV challenge in chickens. The efficacy of rTS-gB in alleviating clinical signs was similar to that of the commercial attenuated ILTV K317 strain. Furthermore, rTS-gB could significantly reduce viral shedding in cloacal and tracheal samples. Our study suggested that the rNDV strain rTS-gB is a thermostable, safe, and highly efficient vaccine candidate against ILT and ND.

Keywords: Newcastle disease virus; glycoprotein gB; infectious laryngotracheitis virus; thermostability; vector vaccine.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antibodies, Viral
Bird Diseases*
Chickens
Herpesvirus 1, Gallid* / genetics
Newcastle Disease* / prevention & control
Newcastle disease virus / genetics

Substances

Antibodies, Viral

Grants and funding

This work was supported by the National Natural Science Foundation of China (grant 31873018), the China Agriculture Research System (grant CARS-41-G11), and the Agricultural Science and Technology Innovation Center Foundation of Hubei (2021-620-000-001-017).