Characterization of Cucurbit Aphid-Borne Yellows Virus (CABYV) from Passion Fruit in Brazil: Evidence of a Complex of Species within CABYV Isolates

Andreza H Vidal; Cristiano Lacorte; Marcio M Sanches; Dione M T Alves-Freitas; Emanuel F M Abreu; Bruna Pinheiro-Lima; Raul C Carriello Rosa; Onildo N Jesus; Magnólia A Campos; Gustavo P Felix; Ana Clara R Abreu; Yam S Santos; Ana Luiza M Lacerda; Arvind Varsani; Fernando L Melo; Simone G Ribeiro

doi:10.3390/v15020410

Characterization of Cucurbit Aphid-Borne Yellows Virus (CABYV) from Passion Fruit in Brazil: Evidence of a Complex of Species within CABYV Isolates

Viruses. 2023 Feb 1;15(2):410. doi: 10.3390/v15020410.

Authors

Andreza H Vidal^{1

2}, Cristiano Lacorte¹, Marcio M Sanches^{1

3}, Dione M T Alves-Freitas¹, Emanuel F M Abreu¹, Bruna Pinheiro-Lima^{1

2}, Raul C Carriello Rosa⁴, Onildo N Jesus⁵, Magnólia A Campos⁶, Gustavo P Felix^{1

7}, Ana Clara R Abreu^{1

7}, Yam S Santos^{1

8}, Ana Luiza M Lacerda¹, Arvind Varsani⁹, Fernando L Melo², Simone G Ribeiro^{1

2}

Affiliations

¹ Embrapa Recursos Genéticos e Biotecnologia, Brasília 70770-917, DF, Brazil.
² Instituto de Ciências Biológicas-IB, PPG BIOMOL, Universidade de Brasília, Brasília 70910-900, DF, Brazil.
³ Embrapa Gado de Corte, Campo Grande 79106-550, MS, Brazil.
⁴ Embrapa Agrobiologia, Seropédica 23891-000, RJ, Brazil.
⁵ Embrapa Mandioca e Fruticultura, Cruz das Almas 44380-000, BA, Brazil.
⁶ Centro de Educação e Saúde, Universidade Federal de Campina Grande, Cuité 58175-000, PB, Brazil.
⁷ Instituto de Ciências Biológicas-IB, Universidade de Brasília, Brasília 70910-900, DF, Brazil.
⁸ Departamento de Microbiologia, Instituto de Biotecnologia Aplicada à Agropecuária, Universidade Federal de Viçosa (UFV), Viçosa 36570-900, MG, Brazil.
⁹ The Biodesign Center for Fundamental and Applied Microbiomics, Center for Evolution and Medicine, School of Life Sciences, Arizona State University, Tempe, AZ 85287, USA.

Abstract

High-throughput sequencing (HTS) has been an important tool for the discovery of plant viruses and their surveillance. In 2015, several virus-like symptoms were observed in passion fruit (PF) plants in Bahia state, Brazil. Using HTS technology, bioinformatics tools, RT-PCR, and Sanger sequencing, we identified the cucurbit aphid-borne yellows virus (CABYV, Polerovirus, Solemoviridae) in co-infection with cowpea aphid-borne mosaic virus (CABMV, Potyvirus, Potyviridae) in PF, in green manure, and spontaneous plants in several localities in Bahia. Complete genomes of CABYV-PF isolates were determined and analyzed with other CABYV isolates available in GenBank that have been identified in various countries. Phylogenetic analysis and pairwise identity comparison with CABYV isolates showed that CABYV-PFs are more closely related to French and Spanish isolates. Overall, analyses of all the CABYV genomes revealed that these could represent ten distinct species, and we thus proposed reclassifying these CABYV as isolates into ten species, tentatively named "Polerovirus curcubitaeprimum" to "Polerovirus curcubitaenonum", and "Polerovirus melo". CABYV-PF is a member of "Polerovirus curcubitaeprimum".

Keywords: CABMV; CABYV; Passiflora; mixed infection; polerovirus.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Brazil
Fruit
Luteoviridae* / genetics
Passiflora*
Phylogeny

Supplementary concepts

Cucurbit aphid-borne yellows virus

Grants and funding

This research was funded by grants from Embrapa, the Conselho Nacional de Desenvolvimento Científico e Tecnológico—CNPq, and the Fundação de Apoio à Pesquisa do Distrito Federal—FAPDF. The Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—CAPES provided scholarships to A.H.V. and B.P.-L., and CNPq did so for D.M.T.A.-F., G.P.F., A.C.R.A. and A.L.M.L.