J Subgroup Avian Leukosis Virus Strain Promotes Cell Proliferation by Negatively Regulating 14-3-3σ Expressions in Chicken Fibroblast Cells

Moyu Wang; Hongmei Li; Xiyu Sun; Jianhua Qiu; Changhua Jing; Huiyue Jia; Yujie Guo; Huijun Guo

doi:10.3390/v15020404

J Subgroup Avian Leukosis Virus Strain Promotes Cell Proliferation by Negatively Regulating 14-3-3σ Expressions in Chicken Fibroblast Cells

Viruses. 2023 Jan 31;15(2):404. doi: 10.3390/v15020404.

Authors

Moyu Wang¹, Hongmei Li¹, Xiyu Sun¹, Jianhua Qiu¹, Changhua Jing¹, Huiyue Jia¹, Yujie Guo¹, Huijun Guo¹

Affiliation

¹ Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, China.

Abstract

This study focuses on clarifying the regulation of chicken 14-3-3σ protein on the fibrous histiocyte proliferation caused by ALV-J-SD1005 strain infection. DF-1 cells were inoculated with 10² TCID₅₀ of ALV-J-SD1005 strain; the cell proliferation viability was dramatically increased and 14-3-3σ expressions were dramatically decreased within 48 h after inoculation. Chicken 14-3-3σ over-expression could significantly decrease the cell proliferation and the ratio of S-phase cells, but increase the ratio of G2/M-phase cells in ALV-J-infected DF-1 cells; by contrast, chicken 14-3-3σ knockdown expression could cause the opposite effects. Additionally, chicken 14-3-3σ over-expression could also dramatically down-regulate the expressions of CDK2/CDC2, but up-regulate p53 expressions in the DF-1 cells; in contrast, the knockdown expression could significantly increase the expressions of CDK2/CDC2 and decrease p53 expressions. It can be concluded that chicken 14-3-3σ can inhibit cell proliferation and cell cycle by regulating CDK2/CDC2/p53 expressions in ALV-J-infected DF1 cells. ALV-J-SD1005 strain can promote cell proliferation by reducing 14-3-3σ expressions. This study helps to clarify the forming mechanism of acute fibrosarcoma induced by ALV-J infection.

Keywords: ALV-J; G2/M-phase block; cell cycle; cell proliferation; chicken 14-3-3σ.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Avian Leukosis Virus* / genetics
Cell Proliferation
Chickens
Fibroblasts
Tumor Suppressor Protein p53 / genetics

Substances

Tumor Suppressor Protein p53

Grants and funding

This study was partially funded by the Project of Shandong Provincial Natural Science Foundation (ZR2020MC179) (ZR2019MC044), the Funds of Shandong Province Modern Agricultural Technology System Innovation Team Program (SDAIT-21-10); the National Key Research and Development Program of China (2016YFD0500800) and the Funds of Shandong “Double Tops” Program.