Nanoparticle surface stabilizing agents influence antibacterial action

Thelma Ameh; Kusy Zarzosa; Jake Dickinson; W Evan Braswell; Christie M Sayes

doi:10.3389/fmicb.2023.1119550

Nanoparticle surface stabilizing agents influence antibacterial action

Front Microbiol. 2023 Feb 9:14:1119550. doi: 10.3389/fmicb.2023.1119550. eCollection 2023.

Authors

Thelma Ameh¹, Kusy Zarzosa^{1

2}, Jake Dickinson¹, W Evan Braswell², Christie M Sayes¹

Affiliations

¹ Department of Environmental Science, Baylor University, Waco, TX, United States.
² United States Department of Agriculture, Animal and Plant Health Inspection Services, Plant Protection and Quarantine, Science and Technology, Insect Management and Molecular Diagnostics Laboratory, Edinburg, TX, United States.

Abstract

The antibacterial properties of nanoparticles are of particular interest because of their potential to serve as an alternative therapy to combat antimicrobial resistance. Metal nanoparticles such as silver and copper nanoparticles have been investigated for their antibacterial properties. Silver and copper nanoparticles were synthesized with the surface stabilizing agents cetyltrimethylammonium bromide (CTAB, to confer a positive surface charge) and polyvinyl pyrrolidone (PVP, to confer a neutral surface charge). Minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and viable plate count assays were used to determine effective doses of silver and copper nanoparticles treatment against Escherichia coli, Staphylococcus aureus and Sphingobacterium multivorum. Results show that CTAB stabilized silver and copper nanoparticles were more effective antibacterial agents than PVP stabilized metal nanoparticles, with MIC values in a range of 0.003 μM to 0.25 μM for CTAB stabilized metal nanoparticles and 0.25 μM to 2 μM for PVP stabilized metal nanoparticles. The recorded MIC and MBC values of the surface stabilized metal nanoparticles show that they can serve as effective antibacterial agents at low doses.

Keywords: alternative antimicrobial resistance agent; engineered nanoparticles; minimum bactericidal concentration; minimum inhibitory concentration; stabilizing agent.