Dataset on estimate of intra-specific genetic variability of African yam bean (Sphenostylis stenocarpa (Hochst. ex A. Rich.) Harms.) based on rbcL gene marker

Jacob Olagbenro Popoola; Davelyne Ifechukude Eruemulor; Omena Bernard Ojuederie; Abiodun Sunday Oyelakin

doi:10.1016/j.dib.2023.108944

Dataset on estimate of intra-specific genetic variability of African yam bean (Sphenostylis stenocarpa (Hochst. ex A. Rich.) Harms.) based on rbcL gene marker

Data Brief. 2023 Feb 2:47:108944. doi: 10.1016/j.dib.2023.108944. eCollection 2023 Apr.

Authors

Jacob Olagbenro Popoola¹, Davelyne Ifechukude Eruemulor², Omena Bernard Ojuederie^{3

4}, Abiodun Sunday Oyelakin⁵

Affiliations

¹ Pure and Applied Biology Programme, College of Agriculture, Engineering and Science (COAES), Bowen University, Iwo, Osun State, Nigeria.
² Department of Biological Sciences, Covenant University, PMB 1023, Ota, Ogun State, Nigeria.
³ Department of Biological Sciences, Biotechnology Unit, Kings University, PMB 555, Ode-Omu, Osun State, Nigeria.
⁴ Food Security and Safety Focus Area, Faculty of Natural and Agricultural Sciences, North-West University, Private Bag X2046, Mmabatho 2735 South Africa.
⁵ Department of Pure and Applied Botany, College of Biosciences, Federal University of Agriculture, Abeokuta (FUNAAB), Ogun State, Nigeria.

Abstract

African yam bean (Sphenostylis stenocarpa (Hochst. ex A. Rich.) Harms.) (Fabaceae) is a versatile crop of nutritional, nutraceutical, and pharmacological value widely grown for its edible seeds and underground tubers. Its high-quality protein, rich mineral elements, and low cholesterol make it a suitable source of food for age groups. However, the crop is still under-exploited and constrained by factors such as intra-specific incompatibility, low yields, indeterminate growth pattern and long gestation period, hard-to-cook (HTC) seeds, and the presence of antinutritional factors (ANFs). To efficiently utilize its genetic resources for improvement and utilization, it is necessary to understand the crop's sequence information and select promising accessions for molecular hybridization trials and conservation purposes. A total of 24 accessions of AYB were collected from the Genetic Resources center of the International Institute of Tropical Agriculture (IITA), Ibadan, Nigeria, and subjected to PCR amplification and Sanger sequencing. The dataset determines genetic relatedness among the twenty-four accessions of AYB. The data consist of partial rbcL gene sequences (24), estimates of intra-specific genetic diversity, the maximum likelihood of transition/transversion bias, and evolutionary relationships based on the UPMGA clustering method. The data identified 13 variables (segregating sites) as SNPs, 5 haplotypes, and codon usage of the species that can be explored further to advance the genetic utilization of AYB.

Keywords: AYB, African yam bean; African yam bean; Breeding; Genetic intra-specific diversity; Germplasm conservation; RbcL, ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit gene.; Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (rbcL) gene.