HS-SPME-GC × GC/MS combined with multivariate statistics analysis to investigate the flavor formation mechanism of tank-fermented broad bean paste

Shiqi Liao; Jinlin Han; Chunyan Jiang; Binbin Zhou; Zhenju Jiang; Jie Tang; Wenwu Ding; Zhenming Che; Hongbin Lin

doi:10.1016/j.fochx.2022.100556

HS-SPME-GC × GC/MS combined with multivariate statistics analysis to investigate the flavor formation mechanism of tank-fermented broad bean paste

Food Chem X. 2022 Dec 27:17:100556. doi: 10.1016/j.fochx.2022.100556. eCollection 2023 Mar 30.

Authors

Shiqi Liao¹, Jinlin Han¹, Chunyan Jiang¹, Binbin Zhou¹, Zhenju Jiang¹, Jie Tang¹, Wenwu Ding^{1

2}, Zhenming Che^{1

2}, Hongbin Lin^{1

2}

Affiliations

¹ School of Food and Bio-engineering, Xihua University, Chengdu 610039, China.
² Chongqing Key Laboratory of Speciality Food Co-Built by Sichuan and Chongqing, Chengdu 610039, China.

Abstract

With the advancement of industrialization, tank fermentation technology is promising for Pixian broad bean paste. This study identified and analyzed the general physicochemical factors and volatile metabolites of fermented broad beans in a thermostatic fermenter. Headspace solid-phase microextraction (HS-SPME)-two-dimensional gas chromatography-mass spectrometry (GC × GC-MS) was applied to detect the volatile compounds in fermented broad beans, while metabolomics was used to explore their physicochemical characteristics and analyze the possible metabolic mechanism. A total of 184 different metabolites were detected, including 36 alcohols, 29 aldehydes, 26 esters, 21 ketones, 14 acids, 14 aromatic compounds, ten heterocycles, nine phenols, nine organonitrogen compounds, seven hydrocarbons, two ethers, and seven other types, which were annotated to various branch metabolic pathways of carbohydrate and amino acid metabolism. This study provides references for subsequent functional microorganism mining to improve the quality of the tank-fermented broad beans and upgrade the Pixian broad bean paste industry.

Keywords: Broad bean paste; GC x GC/MS; Metabolomics; Volatile compounds.