Indoleamine 2,3 dioxygenase 1 immobilization on magnetic nanoparticles for screening inhibitors from coffee

Zhuoting Liu; Yunfang Ping; Lumei Zhang; Jingran Zhang; Ann Van Schepdael; Xu Wang

doi:10.1016/j.fochx.2023.100591

Indoleamine 2,3 dioxygenase 1 immobilization on magnetic nanoparticles for screening inhibitors from coffee

Food Chem X. 2023 Jan 30:17:100591. doi: 10.1016/j.fochx.2023.100591. eCollection 2023 Mar 30.

Authors

Zhuoting Liu¹, Yunfang Ping¹, Lumei Zhang¹, Jingran Zhang², Ann Van Schepdael³, Xu Wang¹

Affiliations

¹ State Key Laboratory of Food Nutrition and Safety, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, China.
² SCIEX, Analytical Instrument Trading Co., Ltd, Beijing 100015, China.
³ Department of Pharmaceutical and Pharmacological Sciences, Pharmaceutical Analysis, KU Leuven - University of Leuven, Leuven, Belgium.

Abstract

In this study, a ligand fishing method was developed to screen potential indoleamine 2,3-dioxygenase 1 (IDO1) inhibitors from coffee extracts by immobilization of IDO1 enzyme on amino-modified magnetic nanoparticles combined with UHPLC-Q-TOF-MS/MS analysis. Parameters including enzyme concentration, immobilization time, the pH of glutaraldehyde and the amount of magnetic nanoparticles were optimized. The results indicated that immobilized IDO1 could be reused 5 times and was stable during storage for 7 days. Several IDO1 ligands were captured by incubating immobilized IDO1 with coffee extract, of which 10 showed an obvious difference comparing to non-conjugated bare nanoparticles. In vitro inhibitory activity was further performed by CE analysis, in which ferulic acid and chlorogenic acid had better IDO1 inhibitory activity, with IC₅₀ value of 113.7 μM and 307.5 μM. These results demonstrate that this method provides an effective platform for identifying and screening IDO1 inhibitors from natural products.

Keywords: 3-O-feruloylquinic acid (PubChem CID 9799386); 5-O-feruloylquinic acid (PubChem CID 73210496); Caffeine (PubChem CID 2519); Chlorogenic acid (PubChem CID 1794427); Coffee; Cryptochlorogenic acid (PubChem CID 9798666); Ferulic acid (PubChem CID 445858); Immobilized IDO1; Isochlorogenic acid A (PubChem CID 6474310); Isochlorogenic acid B (PubChem CID 5281780); Isochlorogenic acid C (PubChem CID 6474309); Ligand fishing; Magnetic nanoparticles; Molecular docking; Neochlorogenic acid (PubChem CID 5280633).