Phase Heterogeneity in Cholesterol-Containing Ternary Phospholipid Lamellar Phases

Deborah L Gater; Keontré I Hughes; Vivian Stojanoff; Abdel F Isakovic

doi:10.1021/acsomega.2c04914

Phase Heterogeneity in Cholesterol-Containing Ternary Phospholipid Lamellar Phases

ACS Omega. 2023 Feb 8;8(7):6225-6233. doi: 10.1021/acsomega.2c04914. eCollection 2023 Feb 21.

Authors

Deborah L Gater¹, Keontré I Hughes^{2

3}, Vivian Stojanoff⁴, Abdel F Isakovic²

Affiliations

¹ University College London, London WC1E 6BT, U.K.
² Colgate University, Hamilton, New York 13346-1338, United States.
³ Michigan State University, East Lansing, Michigan 48824-1312, United States.
⁴ Brookhaven National Laboratory, Upton, New York 11973-5000, United States.

Abstract

Pseudo-ternary mixtures of lamellar phase phospholipids (DPPC and brain sphingomyelin with cholesterol) were studied below T _m while comparing the influence of cholesterol content, temperature, and the presence of small quantities of vitamin D binding protein (DBP) or vitamin D receptor (VDR). The measurements, conducted by X-ray diffraction (XRD) and nuclear magnetic resonance (NMR), cover a range of cholesterol concentrations (20% mol. wt to 40% mol. wt.) and physiologically relevant temperature range (294-314 K). In addition to rich intraphase behavior, data and modeling are used to approximate the lipids' headgroup location variations under the abovementioned experimental conditions.