Application of proteomics to determine the mechanism of ozone on sweet cherries (Prunus avium L.) by time-series analysis

Yuehan Zhao; Zhaohua Hou; Na Zhang; Haipeng Ji; Chenghu Dong; Jinze Yu; Xueling Chen; Cunkun Chen; Honglian Guo

doi:10.3389/fpls.2023.1065465

Application of proteomics to determine the mechanism of ozone on sweet cherries (Prunus avium L.) by time-series analysis

Front Plant Sci. 2023 Feb 9:14:1065465. doi: 10.3389/fpls.2023.1065465. eCollection 2023.

Authors

Yuehan Zhao^{1

2}, Zhaohua Hou³, Na Zhang², Haipeng Ji^{2

4}, Chenghu Dong², Jinze Yu², Xueling Chen⁴, Cunkun Chen², Honglian Guo¹

Affiliations

¹ College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin, China.
² Institute of Agricultural Products Preservation and Processing Technology (National Engineering Technology Research Center for Preservation of Agriculture Product), Tianjin Academy of Agricultural Sciences, Key Laboratory of Postharvest Physiology and Storage of Agricultural Products, Ministry of Agriculture of the People's Republic of China, Tianjin Key Laboratory of Postharvest Physiology and Storage of Agricultural Products, Tianjin, China.
³ College of Food Science and Engineering, Qilu University of Technology (Shandong Academy of Sciences), Ji'nan, China.
⁴ Key Laboratory of Cold Chain Logistics Technology for Agro-product, Ministry of Agriculture and Rural Affairs, Ministry of Agriculture and Rural Affairs, Institute of Agro-Products Processing and Nuclear agricultural Technology, Hubei Academy of Agricultural Sciences, Wuhan, China.

Abstract

This research investigated the mechanism of ozone treatment on sweet cherry (Prunus avium L.) by Lable-free quantification proteomics and physiological traits. The results showed that 4557 master proteins were identified in all the samples, and 3149 proteins were common to all groups. Mfuzz analyses revealed 3149 candidate proteins. KEGG annotation and enrichment analysis showed proteins related to carbohydrate and energy metabolism, protein, amino acids, and nucleotide sugar biosynthesis and degradation, and fruit parameters were characterized and quantified. The conclusions were supported by the fact that the qRT-PCR results agreed with the proteomics results. For the first time, this study reveals the mechanism of cherry in response to ozone treatment at a proteome level.

Keywords: label-free quantification proteomics; mfuzz; ozone; preservation; sweet cherry (Prunus avium L.).

Grants and funding

The present study was supported by Graduate Scientific Research Innovation Project of Tianjin University of Science and Technology in 2021(KYS202157), the National Key R&D Program of China(2018YFF0213605), the Innovation Team of the Tianjin Forestry & Pomology Research System (ITTHRS2021000), Innovative research and experimental projects for young researchers (202009, 2021001); the Major Science and Technology Projects of Shandong Province (2019JZZY20617), Key Laboratory of Storage of Agricultural Products, Ministry of Agriculture and Rural Affairs (kf2020001, Kf2020003, Kf2020005, Kf2020006, kf2021001), The Project Program of Key Laboratory of Tianjin Key Laboratory of Food Quality and Health, China (TJS202101) and Major Innovation Pilot Project of Integration of Science, Education and Industry of Qilu University of Technology (Shandong Academy of Science) (No. 2022JBZ01-08), College Students’ Innovation and Entrepreneurship Training Program of Shandong Province (202210431008).