Biosafety, histological alterations and residue depletion of feed administered anti-parasitic drug emamectin benzoate in golden mahseer, Tor putitora (Hamilton, 1822) as a model candidate fish for sport fishery and conservation in temperate waters

Sumanta Kumar Mallik; Shivam Singh; Neetu Shahi; Prasanna Kumar Patil; Krishna Kala; Richa Pathak; Abhay Kumar Giri; Partha Das; Ritesh Shantilal Tandel; Suresh Chandra; Nanitha Krishna; Rajisha Ravindran; Pramod Kumar Pandey

doi:10.3389/fphar.2023.1106124

Biosafety, histological alterations and residue depletion of feed administered anti-parasitic drug emamectin benzoate in golden mahseer, Tor putitora (Hamilton, 1822) as a model candidate fish for sport fishery and conservation in temperate waters

Front Pharmacol. 2023 Feb 10:14:1106124. doi: 10.3389/fphar.2023.1106124. eCollection 2023.

Affiliations

¹ ICAR-Directorate of Coldwater Fisheries Research (ICAR-DCFR), Bhimtal, India.
² ICAR-Central Institute of Brackishwater Aquaculture (ICAR-CIBA), Chennai, India.
³ ICAR-Central Institute of Fisheries Technology (ICAR-CIFT), Kochi, India.

Abstract

In the present experiment, the attempt has been made to study the biosafety, toxicity, residue depletion and drug tolerance of graded doses of emamectin benzoate (EB) in juveniles of golden mahseer, Tor putitora as a model candidate fish for sport fishery and conservation in temperate waters through an extended medicated feeding. The graded doses of EB viz., 1× (50 μg/kg fish/day), 2 × (100 μg/kg fish/day), 5 × (250 μg/kg fish/day) and 10 × (500 μg/kg fish/day) were administered to golden mahseer juveniles through medicated diet for 21 days at water temperature of 18.6°C. The higher doses of EB did not cause any mortality during and 30 days after the end of medication period, but considerable variations in feeding and behavior were observed. Severe histological alterations observed after EB-diets (5 × and 10×) were vacuolation, pyknotic nuclei, melanomacrophage centre and necrosis in liver; Bowman's capsule dilation, degenerated renal tubules in kidney; myofibril disintegration, muscle oedema, splitting of muscle fibres, migration of inflammatory cells in muscle; and abundant goblet cells, dilated lamina propria and disarrangement of mucosa in intestine tissues. The residual concentrations of EB metabolites Emamectin B_1a and B_1b were analyzed using muscle extracts and were found to be peaked during medication period followed by gradual depletion in post-medication period. The outcome of this study showed that the Emamectin B_1a residual concentration in fish muscle in 1×, 2×, 5×, and 10× EB treatment groups were 1.41 ± 0.49, 1.2 ± 0.7, 9.7 ± 3.3, and 37.4 ± 8.2 μg/kg at 30 days of post-medication period, respectively, which falls under the maximum residue limits (MRLs) of 100 μg/kg. The results support the biosafety of EB at recommended dose of 50 μg/kg fish/day for 7 days. As residue of EB is recorded falling within the MRL, no withdrawal period is recommended for golden mahseer.

Keywords: anti-parasitic drug; emamectin benzoate; golden mahseer; residue depletion; toxicity; withdrawal period.