Soil organic carbon stability mediate soil phosphorus in greenhouse vegetable soil by shifting phoD-harboring bacterial communities and keystone taxa

Shuo Chen; Liying Wang; Shuai Zhang; Naihui Li; Xiaomeng Wei; Yuquan Wei; Lulu Wei; Ji Li; Shaowen Huang; Qing Chen; Tao Zhang; Nanthi S Bolan

doi:10.1016/j.scitotenv.2023.162400

Soil organic carbon stability mediate soil phosphorus in greenhouse vegetable soil by shifting phoD-harboring bacterial communities and keystone taxa

Sci Total Environ. 2023 May 15:873:162400. doi: 10.1016/j.scitotenv.2023.162400. Epub 2023 Feb 24.

Authors

Shuo Chen¹, Liying Wang², Shuai Zhang¹, Naihui Li³, Xiaomeng Wei⁴, Yuquan Wei⁵, Lulu Wei¹, Ji Li⁶, Shaowen Huang⁷, Qing Chen⁸, Tao Zhang¹, Nanthi S Bolan⁹

Affiliations

¹ Beijing Key Laboratory of Farmland Soil Pollution Prevention-control and Remediation, College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, PR China.
² Institute of Agricultural Resources and Environment, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050051, PR China.
³ Department of Horticulture, Northeast Agricultural University, Harbin 150030, PR China.
⁴ College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
⁵ Organic Recycling Institute (Suzhou) of China Agricultural University, Wuzhong District, Suzhou 215128, PR China.
⁶ Beijing Key Laboratory of Farmland Soil Pollution Prevention-control and Remediation, College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, PR China; Organic Recycling Institute (Suzhou) of China Agricultural University, Wuzhong District, Suzhou 215128, PR China.
⁷ Key Laboratory of Plant Nutrition and Fertilizer, Ministry of Agriculture and Rural Affairs/Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China. Electronic address: huangshaowen@caas.cn.
⁸ Beijing Key Laboratory of Farmland Soil Pollution Prevention-control and Remediation, College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, PR China. Electronic address: qchen@cau.edu.cn.
⁹ School of Agriculture and Environment, The University of Western Australia, Perth, WA 6009, Australia; The UWA Institute of Agriculture, The University of Western Australia, Perth, WA 6009, Australia.

PMID: 36842585
DOI: 10.1016/j.scitotenv.2023.162400

Abstract

Addition of organic amendments, such as manure and straw, to arable fields as a partial substitute for mineral phosphorus (P), are a sustainable practice in high-efficiency agricultural production. Different organic inputs may induce varied soil organic carbon (OC) stability and phoD harboring microbes, subsequently regulate P behavior, but the underlying mechanisms are poorly understood. A 11-year field experiment examined P forms by ³¹P-nuclear magnetic resonance (NMR), OC chemical composition by ¹³C NMR, and biologically-based P availability methods, phoD bacterial communities, and their co-occurrence in soils amended with chemical P fertilizer (CF), chemical P partly substituted by organic amendments including pig manure (CM), a mixture of pig manure and corn straw (CMS), and corn straw (CS), with equal P input in all treatments. Organic amendments significantly increased soil labile P_i (CaCl₂-P, citrate-P, 2.91-3.26 and 1.16-1.32 times higher than CF) and P_o (enzyme-P, diesters, 4.08-7.47 and 1.71-2.14 times higher than CF) contents and phosphatase activities, while significantly decreased aromaticity (AI) and recalcitrance indexes (RI) of soil C, compared with CF. The keystone genera in manured soils (Alienimomas and Streptomyces) and straw-applied soils (Janthinobacterium and Caulobacter) were significantly correlated with soil enzyme-P, microbial biomass P (MBP), diesters, and citrate-P. Soil AI and RI were significantly correlated with the phoD keystone and soil P species. It suggested that the keystone was impacted by soil OC stability and play a role in regulating P redistribution in amended soils. This study highlights how manure and straw incorporation altered soil OC stability, shaped the phoD harboring community, and enhanced soil P biological processes promoted by the keystone taxa. The partial substitution of mineral P by mixture of manure and straw is effectively promote soil P availability and beneficial for environmental sustainability.

Keywords: Greenhouse soil; Manure; Soil organic carbon; Soil phosphorus; Straw; phoD gene community.

MeSH terms

Animals
Bacteria
Carbon*
Fertilizers / analysis
Manure
Phosphorus
Soil Microbiology
Soil* / chemistry
Swine
Vegetables

Substances

Soil
Carbon
Phosphorus
Manure
Fertilizers