Tailoring of peroxidase mimetics bifunctional nanocomposite: Dual mode electro-spectroscopic screening of cholesterol and hydrogen peroxide in real food samples and live cells

P Arul; C Nandhini; Sheng-Tung Huang; N S K Gowthaman; Chih-Hung Huang

doi:10.1016/j.foodchem.2023.135747

Tailoring of peroxidase mimetics bifunctional nanocomposite: Dual mode electro-spectroscopic screening of cholesterol and hydrogen peroxide in real food samples and live cells

Food Chem. 2023 Jul 15:414:135747. doi: 10.1016/j.foodchem.2023.135747. Epub 2023 Feb 20.

Authors

P Arul¹, C Nandhini², Sheng-Tung Huang³, N S K Gowthaman⁴, Chih-Hung Huang⁵

Affiliations

¹ Institute of Biochemical and Biomedical Engineering, Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, Taipei 106, Taiwan, ROC. Electronic address: vpsarul@ntut.edu.tw.
² Institute of Biochemical and Biomedical Engineering, Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, Taipei 106, Taiwan, ROC. Electronic address: nandhinisarasu@gmail.com.
³ Institute of Biochemical and Biomedical Engineering, Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, Taipei 106, Taiwan, ROC. Electronic address: ws75624@ntut.edu.tw.
⁴ School of Engineering, Monash University Malaysia, Jalan Lagoon Selatan, 47500 Bandar Sunway, Selangor, Malaysia. Electronic address: gowthaman.nalla@monash.edu.
⁵ Institute of Biochemical and Biomedical Engineering, Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, Taipei 106, Taiwan, ROC. Electronic address: chhuang@ntut.edu.tw.

PMID: 36841102
DOI: 10.1016/j.foodchem.2023.135747

Abstract

A simple and rapid screening of biomarkers in clinical and food matrices is urgently needed to diagnose cardiovascular diseases. The cholesterol (Chol) and hydrogen peroxide (H₂O₂) are critical bio-indicators, which require more inventive detection techniques to be applied to real food, and bio-samples. In this study, a robust dual sensor was developed for Chol and H₂O₂ using hybrid catalyst. Bovine serum albumin (BSA)-capped nanocatalyst was potentially catalyzed 3,3',5,5'-tetramethylbenzidine (TMB), and H₂O₂. The enzymatic nanoelectrocatalyst delivered a wide range of signaling concentrations from 250 nM to 3.0 mM and 100 nM to 10 mM, limit of detection (LOD) of 53.2 nM and 18.4 nM for Chol and H₂O₂. The cholesterol oxidase-BSA-AuNPs-metal-free organic framework (ChOx-BSA-AuNPs-MFOF) based electrode surface effectively operated in live-cells and real-food samples. The enzymatic sensor exhibits adequate recovery of real-food samples (96.96-99.44%). Finally, the proposed system is a suitable choice for the potential applications of Chol and H₂O₂ in clinical and food chemistry.

Keywords: Cholesterol; Electrocatalyst; Enzymatic biosensor; Hydrogen peroxide; Live cells; Whole blood.

MeSH terms

Biosensing Techniques* / methods
Cholesterol
Colorimetry / methods
Coloring Agents
Gold / chemistry
Hydrogen Peroxide / chemistry
Limit of Detection
Metal Nanoparticles* / chemistry
Nanocomposites*
Oxidoreductases
Peroxidase
Peroxidases

Substances

Hydrogen Peroxide
Peroxidase
Gold
Oxidoreductases
Peroxidases
Coloring Agents
Cholesterol