Graphene-encapsulated iron nanoparticles as a non-viral vector for gene delivery into melanoma cells

Magdalena Bamburowicz-Klimkowska; Maciej Malecki; Michal Bystrzejewski; Artur Kasprzak; Ireneusz P Grudzinski

doi:10.1016/j.bbrc.2023.02.042

Graphene-encapsulated iron nanoparticles as a non-viral vector for gene delivery into melanoma cells

Biochem Biophys Res Commun. 2023 Apr 16:652:84-87. doi: 10.1016/j.bbrc.2023.02.042. Epub 2023 Feb 19.

Authors

Magdalena Bamburowicz-Klimkowska¹, Maciej Malecki², Michal Bystrzejewski³, Artur Kasprzak⁴, Ireneusz P Grudzinski⁵

Affiliations

¹ Department of Toxicology and Food Science, Faculty of Pharmacy, Medical University of Warsaw, Banacha 1, 02-097, Warsaw, Poland. Electronic address: mjbamburowicz@wum.edu.pl.
² Department of Applied Pharmacy, Faculty of Pharmacy, Medical University of Warsaw, Banacha 1, 02-097, Warszawa, Poland.
³ Department of Physical Chemistry, Faculty of Chemistry, Warsaw University, L. Pasteura 1, 02-093, Warsaw, Poland.
⁴ Department of Organic Chemistry, Faculty of Chemistry, Warsaw University of Technology, Noakowskiego 3, 00-664, Warsaw, Poland.
⁵ Department of Toxicology and Food Science, Faculty of Pharmacy, Medical University of Warsaw, Banacha 1, 02-097, Warsaw, Poland.

PMID: 36841098
DOI: 10.1016/j.bbrc.2023.02.042

Abstract

The rapid progress of nanotechnology has led to use different nanomaterials for biomedical applications. Among them, graphene-encapsulated magnetic nanoparticles (GEMNS) are recognized as next generation carbon nanomaterials in translation cancer research. In this study, we utilized green fluorescence protein (GFP) expression plasmid DNA (pDNA) and GEMNS decorated with branched polyethyleneimine (PEI) to yield a novel transporter (GEMNS-PEI/pDNA) for gene delivery into melanoma cells (B16F10). The efficiency of transfection was examined using PCR and confocal microscopy. The studies show that the as-designed GEMNS-PEI construct is successfully used to transfect the melanoma cells with pDNA and it should be considered as a potent non-viral vector for introducing naked nucleic acids into eucaryotic cells.

Keywords: Graphene-encapsulated magnetic nanoparticles; Melanoma cells; Non-viral vector; Plasmid DNA; Polyethyleneimine.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA / metabolism
Gene Transfer Techniques
Graphite*
Humans
Iron
Melanoma*
Nanoparticles*
Plasmids
Polyethyleneimine
Transfection

Substances

Graphite
Iron
DNA
Polyethyleneimine