Role of Phytohormones in Biomass and Polyphenol Accumulation in Salvia bulleyana In Vitro Culture

Izabela Grzegorczyk-Karolak; Marta Krzemińska; Anna K Kiss; Aleksandra Owczarek-Januszkiewicz; Monika A Olszewska

doi:10.3390/biom13020227

Role of Phytohormones in Biomass and Polyphenol Accumulation in Salvia bulleyana In Vitro Culture

Biomolecules. 2023 Jan 24;13(2):227. doi: 10.3390/biom13020227.

Authors

Izabela Grzegorczyk-Karolak¹, Marta Krzemińska¹, Anna K Kiss², Aleksandra Owczarek-Januszkiewicz³, Monika A Olszewska³

Affiliations

¹ Department of Biology and Pharmaceutical Botany, Medical University of Lodz, Muszynskiego 1, 90-151 Lodz, Poland.
² Department of Pharmacognosy and Molecular Basis of Phytotherapy, Warsaw Medical University, Banacha 1, Warsaw 02-097, Poland.
³ Department of Pharmacognosy, Medical University of Lodz, Muszynskiego 1, 90-151 Lodz, Poland.

Abstract

Salvia bulleyana is a plant native to the Chinese Yunnan Province. This species has been used in traditional Chinese medicine as a substitute for Danshen (the roots of Salvia miltiorrhiza). The aim of our study was to establish an effective system for propagating S. bulleyana shoots to obtain large amounts of material rich in bioactive compounds. Phytohormones were used to regulate shoot growth and regeneration potential and influence plant secondary metabolism. The shoot tips were incubated on a Murashige and Skoog agar medium supplemented with 0.1 or 0.5 mg/L IAA (indole-3-acetic acid) and the cytokinins benzylaminopurine (BAP), meta-topoline (M-T), 6-benzylaminopurine riboside (RBAP), N-benzyl-9-(2-tetrahydropyranyl)-adenine (BPA) or kinetin, (K) at concentrations of 0.5, 1 or 2 mg/L. It was observed that the type and concentration of growth regulator significantly influenced the regeneration potential of S. bulleyana shoots. The highest multiplication rate was obtained when 0.1 mg/L IAA and 2 mg/L BPA were used. Under these conditions, 100% of shoot tips formed buds and almost seven buds/shoot per explant were obtained after five weeks. Meanwhile, the highest biomass was found for shoots growing on a medium supplemented with 0.1 mg/L IAA and 1 mg/L M-T: 1.2 g of fresh weight and 0.17 g of dry weight. However, a medium with 0.1 mg/L IAA and 2 mg/L RBAP was most favorable for bioactive phenolic acid content, with a total polyphenol level (37.7 mg/g dw) 4.5 times higher than in shoots grown on medium without growth regulators (8.23 mg/g dw). Finally, optimal conditions were selected by TOPSIS (technique for order of preference by similarity to the ideal solution); the culture of S. bulleyana grown on an MS medium containing 0.1 mg/L IAA and 1 mg/L M-T was found to be the most efficient for polyphenol accumulation and can be used for the production of medicinally relevant compounds.

Keywords: N-benzylotetrahydropyranyl adenine; TOPSIS analysis; benzylaminopurine riboside; kinetin; meta-topoline; phytohormones; rosmarinic acid.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biomass
China
Plant Growth Regulators*
Plant Shoots
Polyphenols
Salvia*

Substances

Plant Growth Regulators
Polyphenols

Grants and funding

The work was supported by the Medical University of Lodz, grant Nos. 503/3-022-01/503-31-001 and 503/3-012-01/503-31-001.