Treatment of Inactive Ovaries of Holstein Dairy Cows by Epidural Injection of GnRH Analogue (Receptal) and Its Impact on the Reproductive Hormones, Oxidant/Antioxidant Profile and Micro and Macro-Elements Profile

Yahia A Amin; Alaa Eldin Z Mahmoud; Rana A Ali; Samer S Fouad; Obeid Shanab; Rawia M Ibrahim; Foad Farrag; Mustafa Shukry; Samah F Ibrahim; Liana Fericean; Ragab Hassan Mohamed

doi:10.3390/ani13040653

Treatment of Inactive Ovaries of Holstein Dairy Cows by Epidural Injection of GnRH Analogue (Receptal) and Its Impact on the Reproductive Hormones, Oxidant/Antioxidant Profile and Micro and Macro-Elements Profile

Animals (Basel). 2023 Feb 13;13(4):653. doi: 10.3390/ani13040653.

Authors

Affiliations

¹ Department of Theriogenology, Faculty of Veterinary Medicine, Aswan University, Aswan 81528, Egypt.
² Department of Theriogenology, Faculty of Veterinary Medicine, Sohag University, Sohag 82524, Egypt.
³ Department of Zoology, Faculty of Science, South Valley University, Qena 83523, Egypt.
⁴ Clinical Pathology of Veterinary Medicine, Qena University Hospital, South Valley University, Qena 83523, Egypt.
⁵ Department of Biochemistry, Faculty of Veterinary Medicine, South Valley University, Qena 83523, Egypt.
⁶ Division of Clinical Laboratory Diagnosis, Animal Medicine Department, Faculty of Veterinary Medicine, South Valley University, Qena 83523, Egypt.
⁷ Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh 33516, Egypt.
⁸ Physiology Department, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh 33516, Egypt.
⁹ Department of Clinical Sciences, College of Medicine, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia.
¹⁰ Department of Biology and Plant Protection, Faculty of Agriculture, University of Life Sciences "King Michael I", 300645 Timișoara, Romania.

Abstract

This study was designed to evaluate a new therapeutic approach for inactive ovaries based on the epidural administration of a GnRH agonist (Receptal) and an investigation of the impact of this treatment on the hormonal, oxidant/antioxidant and micro- and macro-element profiles. Sixty cows with postpartum anestrus were divided into two groups: the first group (group R_epid, n = 30) was administered an epidural injection of Receptal, while the second group (group C_epid, n = 30) received saline and was considered the control group. Evaluation of hormonal (progesterone, FSH, LH, testosterone, and cortisol), oxidant/antioxidant (MDA, SOD, GPx and TAC) as well as micro- and macroelement (calcium, phosphorus, manganese and magnesium) profiles was done in serum. The results showed that the epidural injection of Receptal has the potential to induce estrus response and conception incidence in treated cows. Compared to the control group, progesterone, FSH, and LH concentrations were significantly increased in the treated group, whereas testosterone and cortisol decreased (p < 0.05) following treatment. In addition, the treated group had greater TAC and GPx concentrations than the control group. Serum concentrations of magnesium increased (p < 0.05) following receptal treatment, but differences in other minerals were not detected. This research suggests a novel, effective method of treating inactive ovaries with epidural infusion of a GnRH agonist.

Keywords: GnRH analogue; and micro- and macro-elements; epidural injection; inactive ovaries; oxidant/antioxidant profile; reproductive hormones.

Grants and funding

This research was funded by the the Princess Nourah bint Abdulrahman University Researchers Supporting Project number (PNURSP2023R127), Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia; and the project 6PFE of the University of Life Sciences “King Mihai I” from Timisoara and Research Institute for Biosecurity and Bioengineering from Timisoara.