In routine measurements, the length of the analysis time and nfumber of samples analysed during a time unit are crucial parameters, which are especially important for the food analysis, particularly in the case of mycotoxin determinations. High-resolution equipment, including time-of-flight or Orbitrap analyzators, can provide stable instrumental background for high-throughput analyses. In this report, a short, 1 min MS-based multi-mycotoxin method was developed with the application of a short column as a reduced chromatographic separation, taking advantages of the multiplexing and high-resolution capability of the QExactive Orbitrap MS possessing sub-1 ppm mass accuracy. The performance of the method was evaluated regarding selectivity, LOD, LOQ, linearity, matrix effect, and recovery, and compared to a UHPLC-MS/MS method. The final multiplexing method was able to quantify 11 mycotoxins in defined ranges (aflatoxins (corn, 2.8-600 μg/kg; wheat, 1.5-350 μg/kg), deoxynivalenol (corn, 640-9600 μg/kg; wheat, 128-3500 μg/kg), fumonisins (corn, 20-1500 μg/kg; wheat, 30-3500 μg/kg), HT-2 (corn, 64-5200 μg/kg; wheat, 61-3500 μg/kg), T-2 (corn, 10-800 μg/kg; wheat, 4-250 μg/kg), ochratoxin (corn, 4.7-600 μg/kg; wheat, 1-1000 μg/kg), zearalenone (corn, 64-4800 μg/kg; wheat, 4-500 μg/kg)) within one minute in corn and wheat matrices at the MRL levels stated by the European Union.
Keywords: fast mycotoxin measurement; high throughput; parallel ion monitoring multiplexing.