Fe3O4@COF(TAPT-DHTA) Nanocomposites as Magnetic Solid-Phase Extraction Adsorbents for Simultaneous Determination of 9 Mycotoxins in Fruits by UHPLC-MS/MS

Jie Wang; Qingwen Huang; Wenbo Guo; Dakai Guo; Zheng Han; Dongxia Nie

doi:10.3390/toxins15020117

Fe₃O₄@COF(TAPT-DHTA) Nanocomposites as Magnetic Solid-Phase Extraction Adsorbents for Simultaneous Determination of 9 Mycotoxins in Fruits by UHPLC-MS/MS

Toxins (Basel). 2023 Feb 1;15(2):117. doi: 10.3390/toxins15020117.

Authors

Jie Wang^{1

2}, Qingwen Huang², Wenbo Guo², Dakai Guo², Zheng Han², Dongxia Nie^{1

2}

Affiliations

¹ School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China.
² Institute for Agro-Food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China.

Abstract

In this study, a simple and efficient magnetic solid-phase extraction (MSPE) strategy was developed to simultaneously purify and enrich nine mycotoxins in fruits, with the magnetic covalent organic framework nanomaterial Fe₃O₄@COF(TAPT-DHTA) as an adsorbent. The Fe₃O₄@COF(TAPT-DHTA) was prepared by a simple template precipitation polymerization method, using Fe₃O₄ as magnetic core, and 1,3,5-tris-(4-aminophenyl) triazine (TAPT) and 2,5-dihydroxy terephthalaldehyde (DHTA) as two building units. Fe₃O₄@COF(TAPT-DHTA) could effectively capture the targeted mycotoxins by virtue of its abundant hydroxyl groups and aromatic rings. Several key parameters affecting the performance of the MSPE method were studied, including the adsorption solution, adsorption time, elution solvent, volume and time, and the amount of Fe₃O₄@COF(TAPT-DHTA) nanomaterial. Under optimized MSPE conditions, followed by analysis with UHPLC-MS/MS, a wide linear range (0.05-200 μg kg^-1), low limits of detection (0.01-0.5 μg kg^-1) and satisfactory recovery (74.25-111.75%) were achieved for the nine targeted mycotoxins. The established method was further successfully validated in different kinds of fruit samples.

Keywords: UHPLC–MS/MS; covalent organic framework; fruits; magnetic solid-phase extraction; mycotoxins.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adsorption
Chromatography, High Pressure Liquid
Fruit
Limit of Detection
Magnetic Phenomena
Mycotoxins*
Nanocomposites*
Solid Phase Extraction / methods
Tandem Mass Spectrometry

Substances

Mycotoxins
tyrosine-4-azophenyltrimethylammonium chloride

Grants and funding

This work was funded by the Shanghai Agriculture Applied Technology Development Program, China [Grant No.X20210302], the National Natural Science Foundation of China [Grant No. 31972178] and Shanghai Rising-Star Program [Grant No.21QB1403300].