Aggregation-Induced Red Emission Nanoparticle-Based Lateral Flow Immunoassay for Highly Sensitive Detection of Staphylococcal Enterotoxin A

Hanpeng Xiong; Ping Chen; Xirui Chen; Xuanang Shen; Xiaolin Huang; Yonghua Xiong; Yu Su

doi:10.3390/toxins15020113

Aggregation-Induced Red Emission Nanoparticle-Based Lateral Flow Immunoassay for Highly Sensitive Detection of Staphylococcal Enterotoxin A

Toxins (Basel). 2023 Jan 29;15(2):113. doi: 10.3390/toxins15020113.

Authors

Hanpeng Xiong^{1

2}, Ping Chen^{1

2}, Xirui Chen^{1

2}, Xuanang Shen^{1

2}, Xiaolin Huang^{1

2

3}, Yonghua Xiong^{1

2

3}, Yu Su^{1

3}

Affiliations

¹ State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
² Jiangxi-OAI Joint Research Institute, Nanchang University, Nanchang 330047, China.
³ State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Nanchang University, Nanchang 330047, China.

Abstract

Staphylococcal enterotoxin A (SEA) has presented enormous difficulties in dairy food safety and the sensitive detection of SEA provides opportunities for effective food safety controls and staphylococcal food poisoning tracebacks. Herein, a novel aggregation-induced emission (AIE)-based sandwich lateral flow immunoassay (LFIA) was introduced to detect SEA by using red-emissive AIE nanoparticles (AIENPs) as the fluorescent nanoprobe. The nanoprobe was constructed by directly immobilising antibodies on boronate-tagged AIENPs (PBA-AIENPs) via a boronate affinity reaction, which exhibited a high SEA-specific affinity and remarkable fluorescent performance. Under optimal conditions, the ultrasensitive detection of SEA in pasteurised milk was achieved within 20 min with a limit of detection of 0.04 ng mL^-1. The average recoveries of the PBA-AIENP-LFIA ranged from 91.3% to 117.6% and the coefficient of variation was below 15%. It was also demonstrated that the PBA-AIENP-LFIA had an excellent selectivity against other SE serotypes. Taking advantage of the excellent sensitivity of this approach, real chicken and salad samples were further analysed, with a high versatility and accuracy. The proposed PBA-AIENP-LFIA platform shows promise as a potent tool for the identification of additional compounds in food samples as well as an ideal test method for on-site detections.

Keywords: aggregation-induced emission; boronate affinity reaction; lateral flow immunoassay; staphylococcal enterotoxin A.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Enterotoxins / analysis
Gold
Immunoassay / methods
Limit of Detection
Metal Nanoparticles*
Milk / chemistry
Nanoparticles*

Substances

enterotoxin A, Staphylococcal
Enterotoxins
Gold

Grants and funding

This study was supported by the National Natural Science Foundation of China (32160599, 32160598 and 32172296) and the Jiangxi Provincial Natural Science Foundation (20212ACB205011, 20202ACB215004, 20212BAB205032 and 20192BAB204021).