Local irradiation of patient-derived tumors in immunodeficient mice

Amber P van der Zalm; Sanne Bootsma; Hans M Rodermond; Arlene L Oei; Maarten F Bijlsma

doi:10.1016/j.xpro.2023.102098

Local irradiation of patient-derived tumors in immunodeficient mice

STAR Protoc. 2023 Mar 17;4(1):102098. doi: 10.1016/j.xpro.2023.102098. Epub 2023 Feb 7.

Authors

Amber P van der Zalm¹, Sanne Bootsma¹, Hans M Rodermond², Arlene L Oei³, Maarten F Bijlsma¹

Affiliations

¹ Amsterdam UMC Location University of Amsterdam, Center for Experimental and Molecular Medicine, Laboratory of Experimental Oncology and Radiobiology, Meibergdreef 9, 1105AZ Amsterdam, the Netherlands; Oncode Institute, Amsterdam, the Netherlands; Cancer Center Amsterdam, Cancer Biology, De Boelelaan 1118, 1081HV Amsterdam, the Netherlands.
² Amsterdam UMC Location University of Amsterdam, Center for Experimental and Molecular Medicine, Laboratory of Experimental Oncology and Radiobiology, Meibergdreef 9, 1105AZ Amsterdam, the Netherlands; Cancer Center Amsterdam, Cancer Biology, De Boelelaan 1118, 1081HV Amsterdam, the Netherlands; Amsterdam UMC Location University of Amsterdam, Department of Radiation Oncology, Meibergdreef 9, 1105AZ Amsterdam, the Netherlands.
³ Amsterdam UMC Location University of Amsterdam, Center for Experimental and Molecular Medicine, Laboratory of Experimental Oncology and Radiobiology, Meibergdreef 9, 1105AZ Amsterdam, the Netherlands; Cancer Center Amsterdam, Cancer Biology, De Boelelaan 1118, 1081HV Amsterdam, the Netherlands; Amsterdam UMC Location University of Amsterdam, Department of Radiation Oncology, Meibergdreef 9, 1105AZ Amsterdam, the Netherlands. Electronic address: a.l.oei@amsterdamumc.nl.

Abstract

Severe combined immunodeficient mice are typically used for xenografting experiments and show reliable tumor engraftment; however, their Prkd^scid mutation renders them highly sensitive to irradiation. Here, we describe a protocol that allows safe local irradiation of tumor xenografts in immunodeficient mice. We detail the steps for the establishment and handling of patient-derived cancer cultures, subcutaneous injection of cancer cells on the mouse hind limb, localized irradiation in mice, tumor monitoring, and tumor characterization via histological and immunohistochemical assessment. For complete details on the use and execution of this protocol, please refer to Dings et al. (2022).¹.

Keywords: Cancer; Cell Culture; Health Sciences; Microscopy; Model Organisms.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Humans
Mice
Mice, SCID
Neoplasms* / radiotherapy