Transcriptomic Profiling of JEG-3 cells using human leiomyoma derived matrix

Samineh Barmaki; Ahmed Al-Samadi; Katarzyna Leskinen; Wafa Wahbi; Ville Jokinen; Sanna Vuoristo; Tuula Salo; Juha Kere; Satu Wedenoja; Päivi Saavalainen

doi:10.1016/j.bbiosy.2022.100056

Transcriptomic Profiling of JEG-3 cells using human leiomyoma derived matrix

Biomater Biosyst. 2022 Jun 17:7:100056. doi: 10.1016/j.bbiosy.2022.100056. eCollection 2022 Aug.

Authors

Samineh Barmaki¹, Ahmed Al-Samadi², Katarzyna Leskinen³, Wafa Wahbi¹, Ville Jokinen⁴, Sanna Vuoristo⁵, Tuula Salo², Juha Kere^{6

7

8}, Satu Wedenoja^{5

9}, Päivi Saavalainen^{3

7}

Affiliations

¹ Department of Pharmacology, Faculty of Medicine, University of Helsinki, Helsinki 00290, Finland.
² Department of Oral and Maxillofacial Disease, University of Helsinki, Helsinki 00290, Finland.
³ Translational Immunology Research Program, and Department of Clinical and Medical Genetics, University of Helsinki, Helsinki 00290, Finland.
⁴ Department of Chemistry and Materials Science, School of Chemical Engineering, Aalto University, Espoo 00076, Finland.
⁵ Obstetrics and Gynecology, University of Helsinki and Helsinki University Hospital, Helsinki 00290, Finland.
⁶ Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge 14183, Sweden.
⁷ Folkhälsan Research Center, Helsinki 00290, Finland.
⁸ Stem Cells and Metabolism Research Program, University of Helsinki, Helsinki 00014, Finland.
⁹ Stem Cells and Metabolism Research Program, University of Helsinki, and Folkhälsan Research Center, Helsinki 00290, Finland.

Abstract

Oxygen tension varies during placental and fetal development. Although hypoxia drives early trophoblast invasion, low placental oxygen levels during pregnancy show association with pregnancy complications including fetal growth restriction and preeclampsia. JEG-3 cells are often used as a trophoblast model. We studied transcriptional changes of JEG-3 cells on a uterine leiomyoma derived matrix Myogel. This might be the closest condition to the real uterine environment that we can get for an in vitro model. We observed that culturing JEG-3 cells on the leiomyoma matrix leads to strong stimulation of ribosomal pathways, energy metabolism, and ATP production. Furthermore, Myogel improved JEG-3 cell adherence in comparison to tissue culture treated plastic. We also included PDMS microchip hypoxia creation, and observed changes in oxidative phosphorylation, oxygen related genes and several hypoxia genes. Our study highlights the effects of Myogel matrix on growing JEG-3 cells, especially on mitochondria, energy metabolism, and protein synthesis.

Keywords: Biomaterials; Hypoxia; JEG-3; Microenvironment; Microfluidic; Myogel; Oxygen scavenger; PDMS; Placenta; Pre-eclampsia.