Autochthonous bioaugmentation accelerates phenanthrene degradation in acclimated soil

Haiping Gu; Jie Yan; Yuhao Liu; Xuewei Yu; Yan Feng; Xuanyi Yang; Su Shiung Lam; Mu Naushad; Cheng Li; Christian Sonne

doi:10.1016/j.envres.2023.115543

Autochthonous bioaugmentation accelerates phenanthrene degradation in acclimated soil

Environ Res. 2023 May 1:224:115543. doi: 10.1016/j.envres.2023.115543. Epub 2023 Feb 21.

Authors

Haiping Gu¹, Jie Yan¹, Yuhao Liu², Xuewei Yu¹, Yan Feng¹, Xuanyi Yang¹, Su Shiung Lam³, Mu Naushad⁴, Cheng Li⁵, Christian Sonne⁶

Affiliations

¹ School of Forestry, Henan Agricultural University, Zhengzhou 450002, China.
² School of Environmental and Municipal Engineering, North China University of Water Resources and Electric Power, Zhengzhou 450045, China.
³ Higher Institution Centre of Excellence (HICoE), Institute of Tropical Aquaculture and Fisheries (AKUATROP), Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia; Center for Transdisciplinary Research, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, India.
⁴ Department of Chemistry, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.
⁵ School of Forestry, Henan Agricultural University, Zhengzhou 450002, China. Electronic address: licheng@henau.edu.cn.
⁶ Department of Ecoscience, Aarhus University, Frederiksborgvej 399, Roskilde DK-4000, Denmark. Electronic address: cs@ecos.au.dk.

PMID: 36822540
DOI: 10.1016/j.envres.2023.115543

Abstract

Bioaugmentation helps to obtain a microbiome capable of remediating polycyclic aromatic hydrocarbons (PAHs). In this study, acclimation of microorganisms to soil supplemented with phenanthrene (PHE) led to enrichment with PAH-degraders, including those in Actinobacteriota and in the genera Streptomyces, Rhodococcus, Nocardioides, Sphingomonas, and Mycobacterium. Aqueous (28 °C, pH 6.5) and soil cultures inoculated with PHE-acclimated soil showed a high PHE (ca. 50 mg L^-1) degradation efficiency. The PHE degradation kinetics in aqueous and soil incubations fitted to the Gompertz equation and the first-order kinetic equation, respectively. Indigenous microorganisms adapted to PHE in their environment, and this increased their capacity to degrade PHE. The effect of co-contaminants and pathway intermediates on PHE degradation showed that the degradation of PHE improved in the presence of diesel while being hindered by lubricant oil, catechol, salicylic and phthalic acid. Our findings provide theoretical and practical support for bioremediationof PAHs in the environment.

Keywords: Acclimation; Bioaugmentation; Biodegradation; Phenanthrene; Soil.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acclimatization
Biodegradation, Environmental
Phenanthrenes* / metabolism
Polycyclic Aromatic Hydrocarbons* / metabolism
Soil / chemistry
Soil Microbiology
Soil Pollutants* / metabolism

Substances

Soil
Soil Pollutants
phenanthrene
Phenanthrenes
Polycyclic Aromatic Hydrocarbons