Generation of a transparent killifish line through multiplex CRISPR/Cas9mediated gene inactivation

Elife. 2023 Feb 23:12:e81549. doi: 10.7554/eLife.81549.

Abstract

Body pigmentation is a limitation for in vivo imaging and thus for the performance of longitudinal studies in biomedicine. A possibility to circumvent this obstacle is the employment of pigmentation mutants, which are used in fish species like zebrafish and medaka. To address the basis of aging, the short-lived African killifish Nothobranchius furzeri has recently been established as a model organism. Despite its short lifespan, N. furzeri shows typical signs of mammalian aging including telomere shortening, accumulation of senescent cells, and loss of regenerative capacity. Here, we report the generation of a transparent N. furzeri line by the simultaneous inactivation of three key loci responsible for pigmentation. We demonstrate that this stable line, named klara, can serve as a tool for different applications including behavioral experiments and the establishment of a senescence reporter by integration of a fluorophore into the cdkn1a (p21) locus and in vivo microscopy of the resulting line.

Keywords: developmental biology; killifish; mate choice; pigmentation; senescence; transparency.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aging / genetics
  • Animals
  • Clustered Regularly Interspaced Short Palindromic Repeats
  • Fundulidae*
  • Longevity / physiology
  • Mammals / genetics
  • Zebrafish / genetics

Grants and funding

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.