A crystal form of ribulose-1,5-bisphosphate carboxylase/oxygenase from Nicotiana tabacum in the activated state

S W Suh; D Cascio; M S Chapman; D Eisenberg

doi:10.1016/0022-2836(87)90129-x

A crystal form of ribulose-1,5-bisphosphate carboxylase/oxygenase from Nicotiana tabacum in the activated state

J Mol Biol. 1987 Sep 20;197(2):363-5. doi: 10.1016/0022-2836(87)90129-x.

Authors

S W Suh¹, D Cascio, M S Chapman, D Eisenberg

Affiliation

¹ Molecular Biology Institute, University of California, Los Angeles 90024.

PMID: 3681999
DOI: 10.1016/0022-2836(87)90129-x

Abstract

A new crystal form of ribulose-1,5-bisphosphate carboxylase/oxygenase (EC 4.1.1.39) from Nicotiana tabacum has been obtained at alkaline pH with polyethylene glycol 8000 in the presence of a non-ionic detergent, beta-octyl glucoside. The crystals are grown at room temperature by the hanging-drop vapor diffusion technique from a protein solution containing enzyme complexed with CO2, Mg2+, and the transition state analog 2-C-carboxy-D-arabinitol-1,5-bisphosphate. The crystals belong to the the space group P3(1)21 (or P3(2)21) with the cell parameters a = 204.6 A, and c = 117.4 A (1 A = 0.1 nm). The asymmetric unit contains half (L4S4: L, large subunit, 53,000 Mr; S, small subunit, 15,000 Mr) of a hexadecameric molecule (L8S8, 540,000 Mr). The crystals diffract to at least 2.6 A Bragg spacing and are suitable for X-ray structure determination.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Crystallization
Nicotiana / enzymology*
Plants, Toxic*
Ribulose-Bisphosphate Carboxylase* / metabolism

Substances

Ribulose-Bisphosphate Carboxylase

Grants and funding

GM31299/GM/NIGMS NIH HHS/United States