Effects of decoction of Angelica Sinensis, Zingiberis Rhizoma Recens, and mutton on physiology and biochemistry of Sprague Dawley female rats with spleen-kidney Yang deficiency

Zhiyong Liu; Ting Wang; Yu Zhang; Liming Luo; Hong Zhang; Lei Xu; Xianbao Zeng; Zihong Zheng; Zhiyong Liu; Zhengyun Zuo

doi:10.21037/atm-22-6442

Effects of decoction of Angelica Sinensis, Zingiberis Rhizoma Recens, and mutton on physiology and biochemistry of Sprague Dawley female rats with spleen-kidney Yang deficiency

Ann Transl Med. 2023 Jan 31;11(2):91. doi: 10.21037/atm-22-6442.

Authors

Zhiyong Liu^{1

2}, Ting Wang^{1

2}, Yu Zhang^{1

2}, Liming Luo³, Hong Zhang⁴, Lei Xu³, Xianbao Zeng⁴, Zihong Zheng^{1

2}, Zhiyong Liu³, Zhengyun Zuo^{1

2}

Affiliations

¹ School of TCM Under Jiangxi University of Chinese Medicine, Nanchang, China.
² Teaching and Research Office of Basic Theory on TCM in Jiangxi Province, Nanchang, China.
³ Science & Technology Center for Experimental Animal of Jiangxi University of Chinese Medicine, Nanchang, China.
⁴ School of Pharmacy, Jiangxi University of Chinese medicine, Nanchang, China.

Abstract

Background: To examine the effects of each dose of decoction of Angelica sinensis (Dang gui), Zingiberis Rhizoma Recens (Sheng jiang), and mutton (DAZM) on the physiological and biochemical indexes of female rats with spleen-kidney Yang deficiency (SKYD) through 30-day feeding of DAZM, and to evaluate the tonifying effect of DAZM combined with the system of benefit damage index-general score (BDI-GS).

Methods: Sprague Dawley (SD) rats were administered adenine and senna water to establish a SKYD model. The rats were then allocated to 4 groups at random: Model group, and L group, 4.2 g/kg, M group, 8.4 g/kg and H group, 16.8 g/kg. In addition, the group of normal feeding with unlimited diet was set as N group. Blood samples were taken to detect the relevant physiological and biochemical indexes. For organ coefficient analysis, 10 kinds of organ tissues were dissected and weighed. The tonifying effect of DAZM was discussed according to the BDI-GS system.

Results: During the modeling, the weight of rats in the normal group displayed a marked growth trend, and the weight of the model group was markedly lower than that of the normal group. After feeding the rats DAZM at a low, intermediate, and high dose, the anal temperature of rats in each group continued to rise, and finally remained basically the same as that of normal rats. Hematological and urine examinations revealed that the urea nitrogen and creatinine (CRE) of the model group and the experimental group were markedly higher than those of the normal group, and there were marked differences. After intragastric administration of DAZM, E2 increased markedly. The BDI-GS values of the liver, spleen, lung, kidney, brain, ovary, and adrenal gland of female rats in the 3 administration groups of DAZM were >1, and the total cumulative GS value of each organ of female rats was more than 10.

Conclusions: The decoction of DAZM has no obvious effect on the growth, metabolism, and development of female rats with SKYD, causes no obvious damage to organs, and has a certain reparative effect on the kidney damage caused by SKYD.

Keywords: Decoction of Angelica sinensis, Zingiberis Rhizoma Recens, and mutton (DAZM); benefit damage index-general score (BDI-GS); effects of physiology and biochemistry; spleen-kidney Yang deficiency (SKYD).