CRISPR/Cas9-mediated gene editing of vacuolar ATPase subunit d mediates phytohormone biosynthesis and virus resistance in rice

Qinghua Lu; Xiangwen Luo; Xiao Yang; Tong Zhou; Yu Zhang; Ying Lan; Deyong Zhang; Limin Zheng; Yixin Li; Li Li; Songbai Zhang; Yong Liu

doi:10.3389/fpls.2023.1122978

CRISPR/Cas9-mediated gene editing of vacuolar ATPase subunit d mediates phytohormone biosynthesis and virus resistance in rice

Front Plant Sci. 2023 Feb 1:14:1122978. doi: 10.3389/fpls.2023.1122978. eCollection 2023.

Authors

Qinghua Lu¹, Xiangwen Luo^{1

2}, Xiao Yang¹, Tong Zhou³, Yu Zhang², Ying Lan³, Deyong Zhang^{1

2}, Limin Zheng², Yixin Li^{1

2}, Li Li^{1

2}, Songbai Zhang^{1

2}, Yong Liu^{1

2}

Affiliations

¹ Longping Branch of Biology College, Hunan University, Changsha, China.
² State Key Laboratory of Hybird Rice, Hunan Academy of Agricultural Sciences, Changsha, China.
³ Key Laboratory of Food Quality and Safety, Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing, China.

Abstract

Vacuolar ATPases (V-ATPases) are proton pumps for proton translocation across membranes that utilize energy derived from ATP hydrolysis; OsV-ATPase subunit d (OsV-ATPase d) is part of an integral, membrane-embedded V0 complex in the V-ATPase complex. Whether OsV-ATPase d is involved in phytohormone biosynthesis and resistance in rice remains unknown. The knockout mutants of OsV-ATPase d in rice were generated using the CRISPR/Cas9 system, and mutation of OsV-ATPase d did not show any detrimental effect on plant growth or yield productivity. Transcriptomic results showed that OsV-ATPase d is probably involved in mediating the biosynthesis of plant hormones and resistance in rice. Compared to wild type, mutation of OsV-ATPase d significantly increased JA and ABA biosynthesis and resistance against Southern rice black-streaked dwarf virus (SRBSDV), but it decreased resistance against Rice stripe virus (RSV) in rice. The data presented in this study reveal that OsV-ATPase d mediates phytohormone biosynthesis and virus resistance in rice and can be selected as a potential target for resistance breeding in rice.

Keywords: CRISP/Cas9; phytohormone biosynthesis; rice; vacuolar ATPase subunit d; virus resistance.

Grants and funding

This work was supported by the National Natural Science Foundation of China (31501609), and the National Key Research and Development Project (No. 2016YFD0200809).