DNA barcoding detects resurrected taxon Giuris laglaizei (Sauvage 1880) in Sulawesi, Indonesia: Bolano Sau Lake payangka phylogeny, phenotypic characters and implications for Giuris spp. conservation

Samliok Ndobe; Muhammad Saleh Nurdin; Nur Hasanah; Aswad Eka Putra; Kasim Mansyur; Mohamad Nasir; Mashening L Rabuna; Abigail Mary Moore

doi:10.12688/f1000research.108970.2

DNA barcoding detects resurrected taxon Giuris laglaizei (Sauvage 1880) in Sulawesi, Indonesia: Bolano Sau Lake payangka phylogeny, phenotypic characters and implications for Giuris spp. conservation

F1000Res. 2022 Mar 10:11:295. doi: 10.12688/f1000research.108970.2. eCollection 2022.

Authors

Samliok Ndobe¹, Muhammad Saleh Nurdin¹, Nur Hasanah¹, Aswad Eka Putra¹, Kasim Mansyur¹, Mohamad Nasir², Mashening L Rabuna², Abigail Mary Moore³

Affiliations

¹ Faculty of Animal Husbandry and Fisheries, Universitas Tadulako, Palu, Sulawesi Tengah, 94118, Indonesia.
² Parigi Moutong District Marine and Fisheries Service, Petapa, Sulawesi Tengah, 94462, Indonesia.
³ Graduate School, Universitas Hasanuddin, Makassar, Sulawesi Selatan, 90245, Indonesia.

Abstract

Background: The freshwater ichthyofauna of Wallacea is diverse and understudied. A baseline survey of Bolano Sau Lake in Parigi Moutong District, Central Sulawesi Province, Indonesia in 2019 found an eleotrid goby (local name payangka) with characters conforming to the genus Giuris, long considered monophyletic as G. margaritacea/G. margaritaceus but recently found to comprise at least eight species. This study focused on the molecular (DNA barcoding) identification and phenotypic characters of the payangka. Methods: Payangka samples were collected from August to December 2019 in collaboration with local fishermen, weighed and measured, and preserved in 75% ethanol. Length, weight, sex (n=111) and 17 morphometric characters/six meristic counts (n=42) were recorded. DNA barcoding was performed on a fin clipping preserved in 96% ethanol. Homologous nucleotide sequences were obtained from public (GenBank and BOLD) databases, analysis conducted in MEGA X, and phylogenetic trees edited in the Interactive Tree of Life (iToL). Results: Within the deeply divided Giuris clade, the payangka sequence resolved into a sub-clade identified as Giuris laglaizei (Sauvage 1880), a recently resurrected taxon, based on a sequence provided by Philippe Keith. The length-weight relationship (L = 0.0087∙W3.162) indicated mildly allometric positive growth. Size distribution differed significantly between male and female fish with significantly larger mean size of males (13.56 cm) than females (11.62 cm). The meristic formula was: D VI-I,8 A I,8 P 13 V I,5 C15. Phylogenetic analysis indicated four Giuris species in wetlands around Tomini Bay and five in Sulawesi. Conclusions: This first record of G. laglaizei in Indonesia advances knowledge of Wallacean and Indo-Pacific Gobiiformes biogeography and highlights the need for a revision of the conservation status of the taxa currently grouped under Giuris margaritacea/G. margaritaceus in the IUCN Red List and FishBase databases. The data will inform biodiversity and fisheries management at local and regional levels.

Keywords: Eleotridae; Giuris margaritacea; Tomini Bay; Wallacea; amphidromy; meristic; morphometric; phylogeny.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
DNA
DNA Barcoding, Taxonomic
Female
Fishes / genetics
Indonesia
Lakes*
Male
Perciformes* / genetics
Phylogeny

Substances

DNA

Grants and funding

This study was partially supported by the Parigi Moutong District Marine and Fisheries Service, Central Sulawesi under the Bolano Sau Lake Baseline Survey Project in collaboration with Universitas Tadulako.