MnO2@Au nanostructures supported colorimetric biosensing with duplex-specific nuclease-assisted DNA structural transition

Xiaoyi Ma; Wuping Zhou; Haiwen Li; Bo Zhang; Peng Miao

doi:10.1016/j.mtbio.2023.100571

MnO₂@Au nanostructures supported colorimetric biosensing with duplex-specific nuclease-assisted DNA structural transition

Mater Today Bio. 2023 Feb 1:19:100571. doi: 10.1016/j.mtbio.2023.100571. eCollection 2023 Apr.

Authors

Xiaoyi Ma^{1

2}, Wuping Zhou², Haiwen Li², Bo Zhang³, Peng Miao^{1

2

4}

Affiliations

¹ University of Science and Technology of China, Hefei 230026, China.
² Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, China.
³ Department of Radiology, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China.
⁴ Jinan Guokeyigong Science and Technology Development Co., Ltd., Jinan 250103, China.

Abstract

Manganese dioxide (MnO₂) nanosheets are regarded as a new class of two-dimensional nanomaterials with several attractive properties with enormous progress in biomedical fields. Gold nanoparticles (AuNPs) are also important biocompatible nanomaterials with unusual optical properties. Hetero-nanostructure of MnO₂ and AuNPs with the medium of DNA is an interesting topic. In this work, the protection of the hetero-nanostructure from salt-induced aggregation is systematically investigated including the effects of sequence length, reagents concentrations, incubation time and temperature. The MnO₂@Au nanostructures are thus applied for the analysis of miRNA. Duplex-specific nuclease (DSN) catalyzed digestion, hybridization chain reaction (HCR) and catalytic hairpin assembly (CHA) are utilized for signal amplification. By finally analyzing the optical responses of the nanocomponents, highly sensitive analysis of target miRNA can be achieved. Excellent analytical performances are attributed to the unique features of MnO₂@Au nanostructures and signal amplification designs. They are promising basis for the construction of novel biosensors for clinical applications.

Keywords: Catalytic hairpin assembly; DNA nanostructures; Gold nanoparticles; Hybridization chain reaction; MnO2 nanosheets.