Acupuncture regulates the Th17/Treg balance and improves cognitive deficits in a rat model of vascular dementia

Liu Qiuping; Pan Pan; Ling Zhenzhen; Zhang Zhen; Zhang Xuezhu; Li Shuting

doi:10.1016/j.heliyon.2023.e13346

Acupuncture regulates the Th17/Treg balance and improves cognitive deficits in a rat model of vascular dementia

Heliyon. 2023 Jan 31;9(2):e13346. doi: 10.1016/j.heliyon.2023.e13346. eCollection 2023 Feb.

Authors

Liu Qiuping^{1

2

3}, Pan Pan^{1

4}, Ling Zhenzhen^{1

5}, Zhang Zhen^{1

6}, Zhang Xuezhu^{1

2}, Li Shuting^{1

2

3}

Affiliations

¹ First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin, 300380, China.
² National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion, Tianjin, 300380, China.
³ Tianjin University of Traditional Chinese Medicine, Tianjin, 301617, China.
⁴ Yunnan University of Traditional Chinese Medicine, Kunming, Yunnan Province, 650500, China.
⁵ Department of Immune Regulation, Immunology Frontier Research Center, Osaka University, Osaka, Japan.
⁶ Weifang Traditional Chinese Hospital, Shandong Province, 261031, China.

Abstract

Objective: The present study was developed to explore the impact of acupuncture on the Th17/Treg balance in the brain and the periphery and associated changes in cognitive deficits in a rat model of vascular dementia (VD).

Methods: Male Wistar rats (8 weeks old) were randomly assigned to sham-operated (Gs, n = 10), and operation (n = 30) groups. A VD model was established for all rats in the operation group via the permanent bilateral occlusion of the common carotid artery. Behavioral screening of these rats was conducted via a hidden platform trial at 2 months post-operation. These operation group rats were then further subdivided into impaired (Gi) and acupuncture (Ga) groups (n = 10/group). Acupuncture was performed over a 21-day period for rats in the Ga group. A Morris water maze (MWM) test was used to assess cognitive function for rats in all groups. Flow cytometry and fluorescent staining were used to detect Th17 and Treg cells in samples from these animals based on IL-17/FoxP3 or CD4⁺FoxP3⁺/CD4⁺RORγt⁺ staining profiles.

Results: Relative to the Gs group, escape latency values for rats in the Gi group were significantly increased. Following treatment, rats in the Ga group exhibited significant reductions in escape latency values as compared to rats in the Gi group (P < 0.05). The relative Treg proportion in the peripheral blood and spleen additionally trended upwards in these Ga rats as compared to those in the Gi group (P > 0.05), whereas the frequency of Th17 cells in the peripheral blood and spleen of Ga group rats trended downward relative to the Gi group (P > 0.05). Significantly fewer CD4⁺RORγt⁺ and RORγt⁺ cells were detected in the Ga group relative to the Gi group, whereas CD4⁺FoxP3⁺ and FoxP3⁺ cell counts were increased (P < 0.01).

Conclusion: In summary, VD model rats exhibited dysregulated Th17/Treg homeostasis. Acupuncture treatment was sufficient to reduce the frequency and numbers of Th17 cells in these animals while increasing Treg cell levels, thereby alleviating cognitive deficits with respect to both spatial learning and memory impairment. Consequently, the therapeutic benefits of such acupuncture treatment may be attributable to the regulation of the Th17/Treg balance and associated improvements in cognitive function.

Keywords: Acupuncture; Balance; Cognitive deficits; Inflammation; Th17/treg; Vascular dementia.