Deciphering the mitochondrial genome of Juglans mandshurica (Juglandaceae)

Mitochondrial DNA B Resour. 2023 Feb 10;8(2):249-254. doi: 10.1080/23802359.2023.2172974. eCollection 2023.

Abstract

Juglans mandshurica Maxim., 1856 is a second-class, protected, rare tree species of high economic and ecological value. We elucidated the complete mitochondrial (mt) genome of J. mandshurica using the Illumina Novaseq 6000 and Nanopore platforms. The complete sequences of 558,032 and 161,386 bp had an overall GC content of 45.0% and 45.3%, respectively, and 61 genes could be annotated, including 38 protein-coding, 20 tRNA, and 3 rRNA genes. The high-quality J. mandshurica mt genomic sequences presented in this study will serve as a useful resource for a range of genetic, functional, evolutionary, and comparative genomic studies on this species of the Juglandaceae family.

Keywords: Juglans mandshurica; mitochondrial genome; phylogenetic analysis.

Grants and funding

The study was financially supported by Project of Shandong Natural Science Foundation Committee ‘Study on Fragmented Population Characteristics and Genetic Structure of Juglans mandshurica, a National Class II Endangered Plant, in Shandong Province’ (No. ZR2019MC055), the Subject of Key R & D Plan of Shandong Province (Major Scientific and Technological Innovation Project) ‘Mining and Accurate Identification of Forest Tree Germplasm Resources’ (No. 2021LZGC023), Shandong agricultural seed improvement project ‘Collection, preservation and precise identification of germplasm resources of characteristic ecological tree species’ (No. 2019LZGC01805), General survey of herbaceous plant germplasm resources in Shandong Province (No. LCYZ202101), and Wild Plant Conservation and Management Project of ‘Preservation of Germplasm Resources of Chinese Endemic Species of Wild Plants under National Key Protection’ (No. 2019073031).