The complete plastome of Glandora prostrata subsp. lusitanica (Samp.) D.C.Thomas (Boraginaceae), the first chloroplast genome belonging to the Glandora genus

Inês Carvalho Leonardo; Adriana Alberti; France Denoeud; Maria Teresa Barreto Crespo; Jorge Capelo; Frédéric Bustos Gaspar

doi:10.1080/23802359.2023.2175976

The complete plastome of Glandora prostrata subsp. lusitanica (Samp.) D.C.Thomas (Boraginaceae), the first chloroplast genome belonging to the Glandora genus

Mitochondrial DNA B Resour. 2023 Feb 15;8(2):270-273. doi: 10.1080/23802359.2023.2175976. eCollection 2023.

Authors

Inês Carvalho Leonardo^{1

2}, Adriana Alberti³, France Denoeud³, Maria Teresa Barreto Crespo^{1

2}, Jorge Capelo^{4

5}, Frédéric Bustos Gaspar^{1

2}

Affiliations

¹ iBET, Instituto de Biologia Experimental e Tecnológica, Oeiras, Portugal.
² ITQB-NOVA, Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Oeiras, Portugal.
³ Génomique Métabolique, Genoscope, Institut François Jacob, CEA, CNRS, Univ Évry, Université Paris-Saclay, Évry, France.
⁴ ECOCHANGE, CIBIO-InBIO - Research Centre in Biodiversity and Genetic Resources, Universidade do Porto, Vairão, Portugal.
⁵ INIAV, Instituto Nacional de Investigação Agrária e Veterinária I.P., Quinta do Marquês, Oeiras, Portugal.

Abstract

Glandora prostrata (Loisel.) D.C.Thomas (Thomas et al., 2008), besides being a common plant of western and south-western Europe and north-western Africa, is a species with a wealth of reported uses in traditional and folk medicine. The chloroplast genome of Glandora prostrata subsp. lusitanica (Samp.) D.C.Thomas (Thomas et al., 2008) isolate BPTPS049 described in this study is the first publicly available complete plastome belonging to the Glandora genus. The chloroplast genome (GenBank accession number: ON641304) is 150,041 bp in length with 37.5% GC content, displaying a quadripartite structure that contains a pair of inverted repeat regions (25,833 bp each), separated by a large (81,222 bp) and small (17,153 bp) single-copy regions. It has 131 annotated genes including 86 protein-coding genes, 37 tRNA genes, and eight rRNA genes. The phylogenetic analysis performed confirms that G. prostrata subsp. lusitanica is placed under the Boraginaceae family, which belongs to the Boraginales order. This study will contribute to conservation, phylogenetic, and evolutionary studies that comprise this traditional species relevant to the landscape of aromatic, medicinal, and condiment plants from Portugal.

Keywords: Boraginaceae; Glandora prostrata subsp. lusitanica; Illumina MiSeq sequencing; complete chloroplast genome; phylogenetic analysis.

Grants and funding

The work was conducted under the project “barcodingPTplants – DNA barcoding aromatic, medicinal, and condiment plants from Portugal” [02/SAICT/2017 n° 31074] financed by Programa Lisboa 2020, Portugal 2020, and the European Union, through the European Regional Development Fund (ERDF), and with the support of INCD funded by the Foundation for Science and Technology (FCT) and ERDF under the project 01/SAICT/2016 n° 022153. Funding from INTERFACE Programme, through the Innovation, Technology and Circular Economy Fund (FITEC), is gratefully acknowledged, as well as iNOVA4Health [UIDB/04462/2020 and UIDP/04462/2020] and LS4FUTURE [LA/P/0087/2020], programs financially supported by FCT and the Ministério da Ciência, Tecnologia e Ensino Superior (MCTES).