Comparison of Autof Ms1000 and EXS3000 MALDI-TOF MS Platforms for Routine Identification of Microorganisms

Lijuan Xiong; Xu Long; Lijun Ni; Lili Wang; Yang Zhang; Lili Cui; Jian Guo; Chunying Yang

doi:10.2147/IDR.S352307

Comparison of Autof Ms1000 and EXS3000 MALDI-TOF MS Platforms for Routine Identification of Microorganisms

Infect Drug Resist. 2023 Feb 15:16:913-921. doi: 10.2147/IDR.S352307. eCollection 2023.

Authors

Lijuan Xiong^#¹, Xu Long^#¹, Lijun Ni^#², Lili Wang², Yang Zhang¹, Lili Cui¹, Jian Guo², Chunying Yang¹

Affiliations

¹ Department of Laboratory Medicine, the Second Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, Guizhou, People's Republic of China.
² Department of Laboratory Medicine, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, People's Republic of China.

^# Contributed equally.

Abstract

Purpose: Matrix-assisted laser desorption-ionization-time of flight mass spectrometry (MALDI-TOF) has recently been widely used in clinical microbiology laboratories, with the advantages of being reliable, rapid, and cost-effective. Here, we reported the performance of two MALDI-TOF MS instruments, EXS3000 (Zybio, China) and Autof ms1000 (Autobio, China), which are commonly used in clinical microbiology field.

Methods: A total of 209 common clinical common isolates, including 70 gram-negative bacteria strains, 58 gram-positive bacteria strains, 33 yeast strains, 15 anaerobic bacteria strains, and 33 mold strains, and 19 mycobacterial strains were tested. All strains were identified by EXS3000 (Zybio, China) and Autof ms1000 (Autobio, China). Sequence analysis of 16S rRNA or ITS regions was used to verify all strains.

Results: Current study found that species-level discrimination was found to be 191 (91.39%) and 190 (90.91%) by EXS3000 and Autof ms1000, respectively. Genus-level discrimination was 205 (98.09%) by the EXS3000 and 205 (98.09%) by the Autof ms1000, respectively. The correct results at species level of the EXS3000 were 91.43% (64/70) for gram-negative bacteria, 93.1% (54/58) for gram-positive cocci, 93.94% (31/33) for yeast, 100% (15/15) for anaerobes and 81.82% (27/33) for filamentous fungi. The correct results at species level of the Autof ms1000 were 92.86% (65/70) for gram-negative bacteria, 91.38% (53/58) for gram-positive cocci, 93.94% (31/33) for yeast, 100% (15/15) for anaerobes and 78.79% (26/33) for filamentous fungi.

Conclusion: Although the results show that the EXS3000 and Autof ms1000 systems are equally good choices in terms of analytical efficiency for routine procedures, the test result of EXS3000 is slightly better than Autof ms1000. It's worth mentioning that the target plate of the EXS 3000 instrument is reusable, but the target plate of the Autof ms1000 is disposable, making the EXS3000 more effective in reducing costs.

Keywords: Autof ms1000; EXS3000; MALDI-TOF mass spectrometry; anaerobic bacteria; clinical isolates; evaluation; gram-negative bacteria; gram-positive bacteria; identification; mycobacteria; yeast.

Grants and funding

This work was supported by the National Natural Science Foundation [grant number 81971990], Key Discipline of Public Health in Shanghai [grant number GWV-10.1-XK04], Excellent Technology Leader in Shanghai [grant number 20XD1434500].