hPSC-derived lung organoids: Potential opportunities and challenges

Xiaoli Du; Yongpin Dong; Wenfang Li; Yong Chen

doi:10.1016/j.heliyon.2023.e13498

hPSC-derived lung organoids: Potential opportunities and challenges

Heliyon. 2023 Feb 4;9(2):e13498. doi: 10.1016/j.heliyon.2023.e13498. eCollection 2023 Feb.

Authors

Xiaoli Du¹, Yongpin Dong², Wenfang Li², Yong Chen³

Affiliations

¹ Department of Hematology, Guizhou Provincial People's Hospital, Guiyang 550002, China.
² Department of Emergency and Critical Care, Shanghai Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.
³ Central Laboratory, Guizhou Provincial People's Hospital, Guiyang 550002, China.

Abstract

Three-dimensional hPSC-derived lung organoids resemble the fetal lung stage, making them an excellent model for studying human lung development. However, current hPSC-derived lung organoids remain incomplete as they lack native lung components such as vasculature, neurons and immune cells. This highlights the need to generate more complex hPSC-derived lung organoids that can faithfully mimic native human lungs for studying human lung development, regeneration, disease modeling and drug screen. In this review, we will discuss the current studies related to the generation of hPSC-derived lung organoids, highlighting how hPSC-derived lung organoids can contribute to the understanding of human lung development. We further focus on potential approaches to generate more complex hPSC-derived lung organoids containing native cellular components. Finally, we discuss the present limitations and potential applications of hPSC-derived lung organoids in the future.

Keywords: Development; Lung; Neurons; Organoid; Vasculature; hPSC.

Publication types

Review