Bacterial cellulose-based hydrogel with antibacterial activity and vascularization for wound healing

Lili Deng; Yinjun Huang; Shiyan Chen; Zhiliang Han; Zhengzhe Han; Mengtian Jin; Xiangyang Qu; Baoxiu Wang; Huaping Wang; Song Gu

doi:10.1016/j.carbpol.2023.120647

Bacterial cellulose-based hydrogel with antibacterial activity and vascularization for wound healing

Carbohydr Polym. 2023 May 15:308:120647. doi: 10.1016/j.carbpol.2023.120647. Epub 2023 Feb 3.

Authors

Lili Deng¹, Yinjun Huang², Shiyan Chen³, Zhiliang Han¹, Zhengzhe Han⁴, Mengtian Jin¹, Xiangyang Qu¹, Baoxiu Wang¹, Huaping Wang⁵, Song Gu⁶

Affiliations

¹ State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai 201620, PR China.
² Trauma Center, Shanghai General Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 201620, PR China.
³ State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai 201620, PR China. Electronic address: chensy@dhu.edu.cn.
⁴ Department of Orthopedic Surgery and Shanghai Institute of Microsurgery on Extremities, Shanghai Sixth People's Hospital, 600 Yishan Road, Shanghai 200233, PR China.
⁵ State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai 201620, PR China. Electronic address: wanghp@dhu.edu.cn.
⁶ Trauma Center, Shanghai General Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 201620, PR China. Electronic address: song.gu@shgh.cn.

PMID: 36813339
DOI: 10.1016/j.carbpol.2023.120647

Abstract

Skin wounds need an appropriate wound dressing to help prevent bacterial infection and accelerate wound closure. Bacterial cellulose (BC) with a three-dimensional (3D) network structure is an important commercial dressing. However, how to effectively load antibacterial agents and balance the antibacterial activity is a lingering issue. Herein, this study aims to develop a functional BC hydrogel containing silver-loaded zeolitic imidazolate framework-8 (ZIF-8) antibacterial agent. The tensile strength of the prepared biopolymer dressing is >1 MPa, the swelling property is over 3000 %, the temperature can reach 50 °C in 5 min with near-infrared (NIR) and the release of Ag⁺ and Zn²⁺ is stable. In vitro investigation shows that the hydrogel displays enhanced antibacterial activity, and the bacteria survival ratios are only 0.85 % and 0.39 % against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). In vitro cell experiments present that BC/polydopamine/ZIF-8/Ag (BC/PDA/ZIF-8/Ag) shows satisfactory biocompatibility and promising angiogenic ability. In vivo study, the full-thickness skin defect on rats demonstrates remarkably wound healing ability and accelerated skin re-epithelialization. This work presents a competitive functional dressing with effective antibacterial properties and accelerative angiogenesis activities for wound repair.

Keywords: Angiogenesis activity; Antibacterial activity; Bacterial cellulose; Metal-organic framework (MOF); Photothermal; Wound healing.

MeSH terms

Animals
Anti-Bacterial Agents / chemistry
Cellulose / chemistry
Escherichia coli
Hydrogels / chemistry
Rats
Staphylococcal Infections* / drug therapy
Staphylococcus aureus
Wound Healing
Wound Infection* / drug therapy

Substances

Cellulose
Hydrogels
Anti-Bacterial Agents