In vivo osseointegration evaluation of implants coated with nanostructured hydroxyapatite in low density bone

Daniel Almeida; Suelen Cristina Sartoretto; Jose de Albuquerque Calasans-Maia; Bruna Ghiraldini; Fabio Jose Barbosa Bezerra; Jose Mauro Granjeiro; Mônica Diuana Calasans-Maia

doi:10.1371/journal.pone.0282067

In vivo osseointegration evaluation of implants coated with nanostructured hydroxyapatite in low density bone

PLoS One. 2023 Feb 22;18(2):e0282067. doi: 10.1371/journal.pone.0282067. eCollection 2023.

Authors

Daniel Almeida¹, Suelen Cristina Sartoretto², Jose de Albuquerque Calasans-Maia³, Bruna Ghiraldini⁴, Fabio Jose Barbosa Bezerra⁴, Jose Mauro Granjeiro⁵, Mônica Diuana Calasans-Maia⁵

Affiliations

¹ Dentistry School, Universidade Federal Fluminense, Niteroi, RJ, Brazil.
² Department of Oral Surgery, Dentistry School, Universidade Federal Fluminense, Niteroi, RJ, Brazil.
³ Orthodontics Department, Dentistry School, Universidade Federal Fluminense, Niterói, RJ, Brazil.
⁴ Dental Research Division, Dentistry School, Universidade Paulista, São Paulo, SP, Brazil.
⁵ Clinical Research Laboratory, Dentistry School, Universidade Federal Fluminense, Niterói, RJ, Brazil.

Abstract

Objective: This in vivo study, aimed to biomechanically, histomorphometrically and histologically evaluate an implant surface coated with nanostructured hydroxyapatite using the wet chemical process (biomimetic deposition of calcium phosphate coating) when compared to a dual acid-etching surface.

Material and methods: Ten sheep (2-4 years old) received 20 implants, 10 with nanostructured hydroxyapatite coating (HAnano), and 10 with dual acid-etching surface (DAA). The surfaces were characterized with scanning electron microscopy and energy dispersive spectroscopy; insertion torque values and resonance frequency analysis were measured to evaluate the primary stability of the implants. Bone-implant contact (BIC) and bone area fraction occupancy (BAFo) were evaluated 14 and 28 days after implant installation.

Results: The HAnano and DAA groups showed no significant difference in insertion torque and resonance frequency analysis. The BIC and BAFo values increased significantly (p<0.05) over the experimental periods in both groups. This event was also observed in BIC value of HAnano group. The HAnano surface showed superior results compared to DAA after 28 days (BAFo, p = 0.007; BIC, p = 0.01).

Conclusion: The results suggest that the HAnano surface favors bone formation when compared to the DAA surface after 28 days in low-density bone in sheep.

Copyright: © 2023 Almeida et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Coated Materials, Biocompatible / chemistry
Dental Implants*
Durapatite / chemistry
Osseointegration*
Sheep
Surface Properties
Titanium / chemistry

Substances

Durapatite
Coated Materials, Biocompatible
Titanium
Dental Implants

Grants and funding

This study was financed by S.I.N. Implant System, Sao Paulo, Brazil, but the company had no influence in the design, execution, and analysis of the results. The authors report no other potential conflicts of interest for this work. - Initials of the authors who received each award Answer: M.D.C.M. - Grant numbers awarded to each author Answer: 01 (M.D.C.M) - The full name of each funder Answer: SIN Implant System. URL of each funder website Answer: https://www.sinimplantsystem.com.br/ Did the sponsors or funders play any role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript? Answer: NO - The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.