Acupuncture for autophagy in animal models of middle cerebral artery occlusion: A systematic review and meta-analysis protocol

Jingxue Yuan; Ziniu Zhang; Jinxia Ni; Xiaona Wu; Haoyue Yan; Jingni Xu; Qi Zhao; Hongwei Yuan; Lei Yang

doi:10.1371/journal.pone.0281956

Acupuncture for autophagy in animal models of middle cerebral artery occlusion: A systematic review and meta-analysis protocol

PLoS One. 2023 Feb 22;18(2):e0281956. doi: 10.1371/journal.pone.0281956. eCollection 2023.

Authors

Jingxue Yuan¹, Ziniu Zhang¹, Jinxia Ni¹, Xiaona Wu¹, Haoyue Yan¹, Jingni Xu¹, Qi Zhao¹, Hongwei Yuan¹, Lei Yang¹

Affiliation

¹ Department of Acupuncture, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, China.

Abstract

Introduction: Ischemic stroke has high morbidity, disability and mortality rates. The effective treatments recommended by guideline have considerable limitations due to their strict range of adaptation and narrow time window. Acupuncture is an effective and safe treatment for ischemic stroke, and the mechanism may be related to autophagy. In this systematic review, we aim to summarize and evaluate the evidence of autophagy in acupuncture therapy for animal models of middle cerebral artery occlusion (MCAO).

Methods: Publications will be retrieved from the MEDLINE, Embase, Cochrane Library, Web of Science, CNKI, CBM, CVIP and Wanfang databases. We will include animal experimental studies of acupuncture for MCAO, and the control group will receive placebo/sham acupuncture or no treatment after model establishment. Outcome measures must include autophagy and will include neurologic scores and/or infarct size. The Systematic Review Center for Laboratory animal Experimentation (SYRCLE) risk of bias tool will be used to assess the risk of bias. A meta-analysis will be performed if the included studies are sufficiently homogenous. Subgroup analyses will be conducted according to different intervention types and different types of outcomes. Sensitivity analyses will also performed to explore the heterogeneity and to assess the stability of the results. Publication bias will be assessed by funnel plots. The Grading of Recommendations, Assessment, Development, and Evaluation (GRADE) system will be applied to evaluate the quality of evidence in this systematic review.

Discussion: The results of this study may help to explain autophagy in acupuncture therapy for ischemic stroke. The limitation of this review is that all included studies will be retrieved from Chinese or English medical databases due to language barriers.

Registration: We registered in PROSPERO on May 31, 2022. (https://www.crd.york.ac.uk/PROSPERO/display_record.php?RecordID=329917) (CRD42022329917).

Copyright: © 2023 Yuan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acupuncture Therapy* / methods
Animals
Autophagy
Infarction, Middle Cerebral Artery
Ischemic Stroke*
Meta-Analysis as Topic
Models, Animal
Systematic Reviews as Topic

Grants and funding

JXN received the award. This work was supported by the National Natural Science Foundation of China (No.82074527). The funders had and will not have a role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.