Mapping Nucleosome Location Using FS-Seq

Barry Milavetz; Brenna Hanson; Kincaid Rowbotham; Jacob Haugen

doi:10.1007/978-1-0716-2899-7_2

Mapping Nucleosome Location Using FS-Seq

Methods Mol Biol. 2023:2611:21-38. doi: 10.1007/978-1-0716-2899-7_2.

Authors

Barry Milavetz¹, Brenna Hanson², Kincaid Rowbotham², Jacob Haugen²

Affiliations

¹ Department of Biomedical Sciences, School of Medicine, University of North Dakota, Grand Forks, ND, USA. barry.milavetz@UND.edu.
² Department of Biomedical Sciences, School of Medicine, University of North Dakota, Grand Forks, ND, USA.

PMID: 36807061
DOI: 10.1007/978-1-0716-2899-7_2

Abstract

The organization of nucleosomes in eukaryotic chromatin is thought to play a critical role in the regulation of the biological function of the chromatin. Because of this potential role in regulation, a number of techniques have been developed, which combine chromatin fragmentation around nucleosomes with next-generation sequencing to map the location of nucleosomes in chromatin. In this section, a procedure using a kit from New England Biolabs (NEB NEXT Ultra II FS DNA library prep Kit) to fragment chromatin in preparation for next-generation sequencing is described and compared to other available procedures for mapping nucleosome location.

Keywords: Chromatin; NGS; Nucleosomes; Phasing; Sequencing.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Chromatin*
Nucleosomes*

Substances

Nucleosomes
Chromatin

Grants and funding

R03 AI142011/AI/NIAID NIH HHS/United States