Acid-degradable nanocomposite hydrogel and glucose oxidase combination for killing bacterial with photothermal augmented chemodynamic therapy

Dong Dong; Zihao Cheng; Tongyao Wang; Xingyu Wu; Chang Ding; Yong Chen; Huayu Xiong; Jichao Liang

doi:10.1016/j.ijbiomac.2023.123745

Acid-degradable nanocomposite hydrogel and glucose oxidase combination for killing bacterial with photothermal augmented chemodynamic therapy

Int J Biol Macromol. 2023 Apr 15:234:123745. doi: 10.1016/j.ijbiomac.2023.123745. Epub 2023 Feb 17.

Authors

Dong Dong¹, Zihao Cheng², Tongyao Wang³, Xingyu Wu¹, Chang Ding¹, Yong Chen⁴, Huayu Xiong⁵, Jichao Liang⁶

Affiliations

¹ National & Local Joint Engineering Research Center of High-throughput Drug Screening Technology, Hubei Province Key Laboratory of Biotechnology of Chinese Traditional Medicine, State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei University, Wuhan, China.
² College of Pharmacy, Hubei University of Chinese Medicine, Wuhan, China.
³ Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, College of Chemistry and Chemical Engineering, Hubei University, Wuhan, China.
⁴ National & Local Joint Engineering Research Center of High-throughput Drug Screening Technology, Hubei Province Key Laboratory of Biotechnology of Chinese Traditional Medicine, State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei University, Wuhan, China. Electronic address: cy101610@qq.com.
⁵ Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, College of Chemistry and Chemical Engineering, Hubei University, Wuhan, China. Electronic address: xionghuay@126.com.
⁶ National & Local Joint Engineering Research Center of High-throughput Drug Screening Technology, Hubei Province Key Laboratory of Biotechnology of Chinese Traditional Medicine, State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei University, Wuhan, China. Electronic address: liang529114@163.com.

PMID: 36806779
DOI: 10.1016/j.ijbiomac.2023.123745

Abstract

Bacterial infection often delays diabetic wound healing, and even causes serious life-threatening complications. Herein, we successfully developed a Cu₂O/Pt nanocubes-dopping alginate (ALG)- hyaluronic acid (HA) hydrogel (Cu₂O/Pt hydrogel) by simple assembly of the Cu₂O/Pt nanocubes and the ALG-HA mixture. The Cu₂O/Pt hydrogel combined with the glucose oxidase (GOx) can be used for photothermal- and starving-enhanced chemodynamic therapy (CDT) against Gram-negative and Gram-positive bacteria. The GOx can catalyze the glucose to produce gluconic acid and H₂O₂ for starvation therapy, following which the released Cu₂O/Pt nanocubes react with H₂O₂ in the acidic microenvironment to generate highly cytotoxic hydroxyl radicals (·OH) for CDT. Additionally, the Cu₂O/Pt hydrogel can release copper ions gradually with the decrease of pH induced by gluconic acid, which can increase the protein expression and secretion of vascular endothelial growth factor (VEGF) and promote endothelial cell proliferation, migration and angiogenesis, subsequently promoting diabetic wound healing in rats. Our results suggested that the Cu₂O/Pt hydrogel combined with GOx may be a potential therapeutic approach for treating the infected diabetic wound.

Keywords: Chemodynamic therapy; Cu(2)O/Pt hydrogel; Diabetic wound healing; Photothermal therapy.

MeSH terms

Alginates
Animals
Cell Line, Tumor
Glucose Oxidase*
Hyaluronic Acid
Hydrogels / pharmacology
Hydrogen Peroxide
Nanogels
Neoplasms*
Rats
Tumor Microenvironment
Vascular Endothelial Growth Factor A

Substances

gluconic acid
Glucose Oxidase
Nanogels
Hydrogen Peroxide
Vascular Endothelial Growth Factor A
Alginates
Hyaluronic Acid
Hydrogels