Multifunctional two-dimensional Bi2Se3 nanodiscs for anti-inflammatory therapy of inflammatory bowel diseases

Cong Zhang; Qingrong Li; Jie Shan; Jianghao Xing; Xiaoyan Liu; Yan Ma; Haisheng Qian; Xulin Chen; Xianwen Wang; Lian-Ming Wu; Yue Yu

doi:10.1016/j.actbio.2023.02.016

Multifunctional two-dimensional Bi₂Se₃ nanodiscs for anti-inflammatory therapy of inflammatory bowel diseases

Acta Biomater. 2023 Apr 1:160:252-264. doi: 10.1016/j.actbio.2023.02.016. Epub 2023 Feb 17.

Authors

Cong Zhang¹, Qingrong Li², Jie Shan³, Jianghao Xing⁴, Xiaoyan Liu⁵, Yan Ma⁶, Haisheng Qian⁶, Xulin Chen³, Xianwen Wang⁷, Lian-Ming Wu⁸, Yue Yu⁹

Affiliations

¹ Division of Gastroenterology, Division of Life Science and Medicine, The First Affiliated Hospital of USTC, University of Science and Technology of China, Hefei, Anhui 230026, China; School of Biomedical Engineering, Research and Engineering Center of Biomedical Materials, Anhui Provincial Institute of Translational Medicine, Anhui Medical University, Hefei 230032, China.
² Department of Pharmacology, School of Basic Medical Sciences, Anhui Medical University, Hefei 230032, China.
³ Department of Burns, The First Affiliated Hospital of Anhui Medical University, Hefei 230032, China.
⁴ Department of Oncology, The First Affiliated Hospital of Anhui Medical University, Hefei 230032, China.
⁵ Division of Gastroenterology, Division of Life Science and Medicine, The First Affiliated Hospital of USTC, University of Science and Technology of China, Hefei, Anhui 230026, China.
⁶ School of Biomedical Engineering, Research and Engineering Center of Biomedical Materials, Anhui Provincial Institute of Translational Medicine, Anhui Medical University, Hefei 230032, China.
⁷ School of Biomedical Engineering, Research and Engineering Center of Biomedical Materials, Anhui Provincial Institute of Translational Medicine, Anhui Medical University, Hefei 230032, China. Electronic address: xianwenwang@ahmu.edu.cn.
⁸ Department of Radiology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China. Electronic address: wlmssmu@126.com.
⁹ Division of Gastroenterology, Division of Life Science and Medicine, The First Affiliated Hospital of USTC, University of Science and Technology of China, Hefei, Anhui 230026, China. Electronic address: yuyuemd@ustc.edu.cn.

PMID: 36805534
DOI: 10.1016/j.actbio.2023.02.016

Abstract

The overexpression of reactive oxygen and nitrogen species (RONS) in the colonic mucosa destroys the mucosa and its barrier, accelerating the occurrence of inflammatory bowel disease (IBD). The elimination of RONS from the inflammatory colon has proven effective in alleviating IBD. Although many nanoantioxidants have been developed, preparing robust and efficient nano-antioxidants remains challenging. Herein, by modifying bismuth selenide (Bi₂Se₃) nanodiscs with polyvinylpyrrolidone (PVP), a multifunctional nanozyme based on 2D nanomaterials was developed for the treatment of IBD. By eliminating multiple RONS, such as hydroxyl radicals (•OH), superoxide anions (O₂^-^•), nitric oxide (NO), and Bi₂Se₃ nanodiscs enhanced cellular survival after H₂O₂ stimulation. As evidenced by colonic injury, reduced body weight, spleen index, and proinflammatory cytokine levels in mice, RONS clearance alleviated intestinal inflammation in a prevention and delay model of acute colitis. 16S rDNA amplicon sequencing reveals that Bi₂Se₃ nanodiscs had the potential to regulate intestinal flora, increase the proportion of Firmicutes to Bacteroidetes, inhibit Proteobacteria bacteria, and restore intestinal homeostasis. This study highlights the use of Bi₂Se₃ nanodiscs with excellent biocompatibility, multienzyme functionality, and RONS scavenging ability as treatments for IBD without apparent adverse effects. STATEMENT OF SIGNIFICANCE: RONS were efficiently scavenged by Bi₂Se₃ nanodiscs. Bi₂Se₃ nanodiscs could be as a promising and potentially safe theraeputic agent for IBD. The gut microbiota could be modulated by Bi₂Se₃ nanodiscs.

Keywords: Bi(2)Se(3); Free radical scavenging; Inflammatory bowel disease; Nanozymes; Reactive oxygen species.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Anti-Inflammatory Agents / pharmacology
Colitis* / prevention & control
Colon
Dextran Sulfate / therapeutic use
Disease Models, Animal
Hydrogen Peroxide
Inflammatory Bowel Diseases* / drug therapy
Mice
Reactive Oxygen Species

Substances

Hydrogen Peroxide
Reactive Oxygen Species
Anti-Inflammatory Agents
Dextran Sulfate