Objective: The antifriction and antiwear effects of gelatin nanoparticles (GLN-NP) on artificial joint materials in bionic joint lubricant were investigated to provide a theoretical basis for the development of new bionic joint lubricant.
Methods: GLN-NP was prepared by cross-linking collagen acid (type A) gelatin with glutaraldehyde by acetone method, and the particle size and stability of GLN-NP were characterized. The biomimetic joint lubricants with different concentrations were prepared by mixing 5, 15, and 30 mg/mL GLN-NP with 15 and 30 mg/mL hyaluronic acid (HA), respectively. The friction reduction and antiwear effects of the biomimetic joint lubricants on zirconia ceramics were investigated on a tribometer. The cytotoxicity of each component of bionic joint lubricant on RAW264.7 mouse macrophages was evaluated by MTT assay.
Results: The particle size of GLN-NP was about 139 nm, and the particle size distribution index was 0.17, showing a single peak, indicating that the particle size of GLN-NP was uniform. In complete culture medium, pH7.4 PBS, and deionized water at simulated body temperature, the particle size of GLN-NP did not change more than 10 nm with time, indicating that GLN-NP had good dispersion stability and did not aggregate. Compared with 15 mg/mL HA, 30 mg/mL HA, and normal saline, the friction coefficient, wear scar depth, width, and wear volume were significantly reduced by adding different concentrations of GLN-NP ( P<0.05); there was no significant difference between different concentrations of GLN-NP ( P>0.05). Biocompatibility test showed that the cell survival rate of GLN-NP, HA, and HA+GLN-NP solution decreased slightly with the increase of concentration, but the cell survival rate was more than 90%, and there was no significant difference between groups ( P>0.05).
Conclusion: The bionic joint fluid containing GLN-NP has good antifriction and antiwear effect. Among them, GLN-NP saline solution without HA has the best antifriction and antiwear effect.
目的: 探讨明胶纳米粒（gelatin nanoparticle，GLN-NP）在仿生关节润滑液中对人工关节材料的减摩、抗磨作用，为发展新型仿生关节润滑液提供理论基础。.
方法: 采用丙酮法将胶原蛋白酸性（A型）明胶经戊二醛交联得到GLN-NP，表征GLN-NP粒径大小及稳定性。将5、15、30 mg/mL GLN-NP分别与15、30 mg/mL透明质酸（hyaluronic acid，HA）复合，制备不同浓度仿生关节润滑液，并用摩擦试验机观察其对氧化锆陶瓷的减摩和抗磨作用。应用MTT法考察仿生关节润滑液各成分对RAW264.7小鼠巨噬细胞的毒性。.
结果: GLN-NP 粒径约为139 nm，粒径分布指数为0.17，表现为单一的高峰，说明GLN-NP颗粒粒径均一。在模拟体温条件下的完全培养基、pH7.4 PBS和去离子水中，GLN-NP随时间变化的粒径变化不超过10 nm，说明GLN-NP有很好的分散稳定性，且未发生聚集现象。与单纯15、30 mg/mL HA及生理盐水相比，在其中添加不同浓度GLN-NP后，其摩擦系数明显降低，磨痕深度、宽度及磨损体积均明显减小（ P<0.05）；各浓度GLN-NP组间差异无统计学意义（ P>0.05）。生物相容性检测示，GLN-NP、HA和HA+GLN-NP溶液随浓度提高细胞存活率稍有下降，但细胞成活率均在90%以上，组间差异无统计学意义（ P>0.05）。.
结论: 制备的含GLN-NP仿生关节润滑液具有良好的减摩、抗磨作用；其中不含HA的GLN-NP生理盐水溶液减摩、抗磨效果最好。.
Keywords: Gelatin nanoparticle; antifriction; antiwear; artificial joint material; bionic joint lubricant.