Fungal aerosols in rabbit breeding environment: Metagenetic insight into PM2.5 based on third-generation sequencing technology

Zhicheng Song; Yumei Cai; Gongyan Liu; Guanliu Yu

doi:10.1016/j.envres.2023.115480

Fungal aerosols in rabbit breeding environment: Metagenetic insight into PM_2.5 based on third-generation sequencing technology

Environ Res. 2023 May 1:224:115480. doi: 10.1016/j.envres.2023.115480. Epub 2023 Feb 15.

Authors

Zhicheng Song¹, Yumei Cai², Gongyan Liu³, Guanliu Yu⁴

Affiliations

¹ College of Life Sciences, Shandong Normal University, Jinan, 250014, China.
² College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an, 271018, China.
³ Institute of Animal Husbandry and Veterinary, Shandong Academy of Agricultural Sciences, Jinan, 251000, China.
⁴ College of Life Sciences, Shandong Normal University, Jinan, 250014, China. Electronic address: yuguanliu@sdnu.edu.cn.

PMID: 36796612
DOI: 10.1016/j.envres.2023.115480

Abstract

Fungal aerosols are a vital environmental hazard factor impeding the development of the rabbit breeding industry and threatening public health. This study aimed to determine fungal abundance, diversity, composition, diffusion, and variability in aerosols in rabbit breeding environments. Twenty PM_2.5 filter samples were collected from five sampling sites (i.e. En5, In, Ex5, Ex15, and Ex45) in a modern rabbit farm in Linyi City, China. Fungal component diversity at the species level was analyzed in all samples using third-generation sequencing technology. Results revealed that fungal diversity and community composition in PM_2.5 significantly differed across different sampling sites, and different pollution levels. The highest concentrations of PM_2.5 and fungal aerosols (i.e., 102.5 μg/m³ and 18.8 × 10³ CFU/m³, respectively) were found at Ex5, and these concentrations were found to decrease as the distance from the exit increased. However, no significant correlation was observed between the internal transcribed spacer (ITS) gene abundance and overall PM_2.5 levels, except for Aspergillus ruber and Alternaria eichhorniae. Although most fungi are not pathogenic to humans, zoonotic pathogenic microorganisms that cause pulmonary aspergillosis (e.g., Aspergillus ruber) and invasive fusariosis (e.g., Fusarium pseudensiforme) were observed. The relative abundance of A. ruber was higher at Ex5 than that at In, Ex15, and Ex45 (p < 0.01), and the relative abundance of the fungal species decreased with an increase in distance from the rabbit houses. Moreover, four potential novel strains of Aspergillus ruber were discovered, with 82.9%-90.3% of the nucleotide and amino acid sequences similar to those of reference strains. This study highlights the importance of rabbit environments as a source in shaping fungal aerosol microbial communities. To the best of our knowledge, this is the first study to uncover the initial characteristics of fungal biodiversity and dispersion of PM_2.5 in rabbit breeding environments, contributing to infectious disease control and prevention in rabbits.

Keywords: Environmental microbiology; Fungal aerosol; PM(2.5); Rabbit house; Third-generation sequencing.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aerosols / analysis
Air Microbiology
Air Pollutants* / analysis
Animals
Breeding
Environmental Monitoring / methods
Fungi
Humans
Particulate Matter* / analysis
Rabbits
Technology

Substances

Particulate Matter
Aerosols
Air Pollutants

Supplementary concepts

Aspergillus ruber